TNF-α,NF-KBp65,PAI-1在妊娠期糖尿病孕妇血清及胎盘组织中的表达变化及其意义  

Expression of TNF-α, NF-KBp65 and PAI-1 in Serum and Placental Tissue of Gestational Diabetes Pregnant Women

作　　者：王少丽 董晋 张明 吕鑫荣 白金博 

机构地区：[1]西北妇女儿童医院,陕西西安717600

出　　处：《现代生物医学进展》2016年第25期4867-4869,4858,共4页Progress in Modern Biomedicine

摘　　要：目的:探究TNF-α、NF-KBp65以及PAI-1在妊娠期糖尿病孕妇血清和胎盘组织中的表达变化及其临床意义。方法:选取自47例定期产检并且住院分娩的妊娠期糖尿病孕妇作为观察组,再选取同期分娩的47例正常孕妇作为对照组,比较两组孕妇血清和胎盘组织中TNF-α、NF-KBp65和PAI-1的表达水平,并分析血清中TNF-α、NF-KBp65、PAI-1表达水平的相关性。结果:观察组孕妇血浆的TNF-α和PAI-1表达水平明显较高,差异具有统计学意义(P均<0.001)。妊娠期糖尿病患者血浆中PAI-1表达水平和TNF-α表达水平呈正相关关系(r=0.843,P<0.001)。观察组孕妇的空腹-OGTT、1h-OGTT、2h-OGTT、空腹胰岛素、空腹血糖以及胰岛素抵抗指数均明显高于对照组(P均<0.001)。胰岛素抵抗指数与妊娠期糖尿病患者血浆中TNF-α的表达水平相关性最高(Beta=0.769);口服葡萄糖耐受实验中1-h的血糖水平与PAI的表达水平相关性最高(Beta=0.637)。观察组孕妇的TNF-α、NF-KBp65和PAI-1的阳性率显著高于对照组,差异具有统计学意义(P<0.001,=0.039,0.007)。结论:血清和胎盘组织中的TNF-α、NF-KBp65和PAI-1可能参与了妊娠期糖尿病的发生与发展。Objective： To investigate the expression changes of TNF-α, NF-KBp65 and PAI-1 in serum and placental tissue of gestational diabetes pregnant women, as well as its clinical significance. Methods： Select 47 regular prenatal and hospital childbirth gestational diabetes pregnant women as observation group, and select 47 cases of normal pregnant women in childbirth at the same period as the control group. Compare the expression levels of TNF-α, NF-KBp65 and PAI-1 in serum and placental tissue between the two groups,and analyze the correlation of the expression levels of serum TNF-α, NF-KBp65 and PAI-1. Results： The expression levels of serum TNF-α and PAI-1 were higher in pregnant women of observation group, with statistically significant difference（P〈0.001）. In plasma of patients with gestational diabetes, the expression levels of PAI-1 were positively correlated with the TNF-α expression levels（r=0.843,P〈0.001）. In observation group, the empty stomach-OGTT, 1 h-OGTT, 2h-OGTT, fasting insulin, fasting plasma glucose and insulin resistance indexes were significantly higher than in control group（P〈0.001）. Insulin resistance index had the highest correlation with the expression levels of TNF-α in plasma of patients with gestational diabetes（Beta = 0.769）. Oral glucose tolerance test, the 1-h blood glucose levels had the highest correlation with the expression level of PAI（Beta=0.637）. In observation group, the pregnant women had higher positive rates of TNF-α, NF-KBp65 and PAI 1 than in control group, and differences had statistical significance（P〈0.001=0.039,0.001）. Conclusion： TNF-α, NF-KBp65 and PAI-1 in the serum and placental tissues might participate in the onset and development of gestational diabetes mellitus.

关 键 词：TNF-Α NF-KBP65 PAI-1 妊娠期糖尿病 酶联免疫吸附试验 

分 类 号：R714.256[医药卫生—妇产科学]