白桦BpLHY、BpTOC1与BpGI节律基因生物信息学及表达分析  

Bioinformatics and Expression Analysis of Circadian Related Gene BpLHY、BpTOC1 and BpGI in Betula platyphylla Suk.

作　　者：孙丰坤[1] 周姗[1] 李蕾蕾[1] 詹亚光[1,2] 曾凡锁[1,2] 

机构地区：[1]东北林业大学生命科学学院 [2]林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《林业科学研究》2016年第5期638-645,共8页Forest Research

基　　金：中央高校基本科研业务费专项资金项目(2572014DA04);国家自然科学基金项目(J1210053和31200463);黑龙江省博士后启动基金(LBH-Q12166)资助

摘　　要：[目的]探究白桦生物钟基因运行机制及在生长发育、胁迫应答中的作用。[方法]运用生物信息学方法对白桦BpLHY、BpTOC1、BpGI节律基因进行分析,预测其编码蛋白的结构功能。利用RT-PCR方法检测白桦BpLHY、BpTOC1、BpGI基因昼夜表达模式,在非生物胁迫重金属Cd、低温(4℃)、与盐(Na Cl)及信号诱导SA(水杨酸)、SNP(硝普钠)下的表达情况。[结果]白桦BpLHY、BpTOC1、BpGI节律基因编码的均为疏水性非分泌型,具有跨膜能力的mixed蛋白。白桦BpTOC1、BpLHY基因表达量均呈现白天低夜晚高的昼夜变化模式,而BpGI表达量则表现出白天高夜晚低的模式。在重金属Cd、低温(4℃)、与盐(Na Cl)非生物胁迫下,BpLHY、BpTOC1与BpGI均上调表达。SA、SNP诱导白桦BpLHY与BpTOC1表达下调,而诱导BpGI表达上调。[结论]白桦BpLHY、BpTOC1、BpGI节律基因昼夜表达模式及非生物胁迫、信号诱导下的表达特征为进一步研究白桦生物钟基因作用机制及其在植物生长发育和胁迫应答中的作用奠定了基础。[ Objective ] To clarify the mechanism and functions in regulating plant growth and stress response of cir- cadian clock genes in Betula platyphylla Suk. [ Method] The sequences of BpLHY, BpTOC1 and BpGI were ana- lyzed, and the structure and functions of coding proteins were predicted by bioinformatics method. The expression levels of BpLHY, BpTOC1 and BpGI were quantified using qRT-PCR. [ Result]BpLHY, BpTOC1 and BpGI genes in B. platyphylla were 2 283bp, 1 623bp, and 3 507bp, encoding 760, 540, and 1 168 amino acids, respectively. The proteins encoded by these genes were hydrophobic, non-secreted transmembrane protein. BpTOC1 and BpLHY transcript levels were low during the daytime, but high at night. In contrast, BpGI expression was low during the daytime, and high at night and its highest expression levels increased by 29.28 times at 9：00 am. The expressions of BpLHY, BpTOC1 and BpGI increased by 105% to 2 390% in the abiotie stress of heavy metal cadmium, low ternperature （4℃）, salt （NaC1） ; SA and SNP induced the transcription levels of BpLHY, BpTOC1 genes reducing by 70.1% to 97.75% while the expression of BpGI increasing by 150% to 280%. [ Conclusion] This study may pro- vide some references for further study of BpLHY, BpTOC1 and BpGI function in regulation of plant growth and stress response and the mechanism of circadian clock in B. platyphylla.

关 键 词：白桦 节律基因 生物信息学 非生物胁迫 

分 类 号：S792.153[农业科学—林木遗传育种]