镇肝熄风汤对帕金森病肝阳上亢证大鼠中脑Akt和GSK-3β磷酸化的影响  

Effects of Zhengan Xifeng Decoction on the Phosphorylation of Akt and GSK-3β in the Midbrain in Rats with Parkinson's Disease and the Syndrome of Liver-Yang Hyperactivity

作　　者：王晓丽[1] 孙影[2] 朱兰芹[2] 綦艳秋[2] 张丽华[2] 张红宇[2] 董妙先[2] 

机构地区：[1]齐齐哈尔医学院药学院,齐齐哈尔161006 [2]齐齐哈尔医学院卫生所,齐齐哈尔161006

出　　处：《世界科学技术-中医药现代化》2016年第8期1374-1378,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81373629):基于PI3K/Akt/GSK-3β信号通路的镇肝熄风法抑制帕金森病氧化应激损伤的机制研究;负责人:董妙先

摘　　要：目的:本研究主要观察镇肝熄风汤对帕金森病肝阳上亢证大鼠中脑Akt及其下游靶分子GSK-3β磷酸化的影响。方法:105只大鼠随机分为对照组、假手术组、模型组、镇肝熄风汤高、中、低剂量组和司来吉兰组。采用6-OHDA中脑微量注射合并附子汤灌胃制备帕金森病肝阳上亢证模型,采用分光光度法检测中脑组织caspase-3活性,Western blot检测脑组织Akt和GSK-3β磷酸化水平。结果:与假手术组比较,帕金森病肝阳上亢证大鼠中脑caspase-3活性明显增强,Akt和GSK-3β磷酸化水平明显升高,而镇肝熄风汤组中脑caspase-3活性明显减弱,Akt和GSK-3β磷酸化水平明显升高。结论:镇肝熄风汤能够减弱帕金森病肝阳上亢证大鼠中脑caspase-3活性,其机制可能与Akt活化和GSK-3β失活有关。The aim of this study was to investigate the effects of Zhengan Xifeng decoction （ZGXFD） on the phosphorylation of Akt and its downstream target molecule GSK-3β in the midbrain of Parkinson＇ s disease （PD） rats with the syndrome of liver-yang hyperactivity. A total of 105 pathogen-free male Wistar rats were randomly divided into seven groups： the control group, the sham-operated group, the model group, the ZGXFD high- , medium- and low-dose groups and the selegiline group. Microinjeetion of 6-OHDA into substantia nigra and the intragastric administration of Fuzi decoction were given to establish the rat model of Parkinson＇ s disease with syndrome of liver-yang hyperactivity. The activity of caspase-3 was detected using spectrophotometry. The phosphorylation of Akt and GSK-3β in the midbrain was analyzed by western blot. It was found that the activity of caspase-3 in the midbrain was enhanced while the phosphorylation of Akt and GSK-3β was increased in the PD rats of the model group compared with the sham-operated group. The activity of caspase-3 in the midbrain was inhibited and the phosphorylation of Akt and GSK-3β was up-regulated in the ZGXFD groups. In conclusion, it was demonstrated that the anti-apoptotic effects of ZGXFD may activate the Akt and suppress of the activity of GSK-3β in the midbrain of PD rats with the syndrome of liver-yang hyperactivity.

关 键 词：肝阳上亢证 镇肝熄风汤 帕金森病 半胱天冬酶3 糖原合成酶激酶-3Β 

分 类 号：R2-031[医药卫生—中西医结合]