新生大鼠手术切口对脊髓背角BDNF含量的影响  

EFFECTS OF NEONATAL HIND PAW INCISION ON THE EXPRESSION OF BDNF IN THE SPINAL DORSAL HORN

作　　者：丁绪 李松[2] 蔡捷[2] 邰隽[3] 邢国刚[2] 

机构地区：[1]首都医科大学附属北京儿童医院,北京市儿科研究所营养研究室,北京100045 [2]北京大学神经科学研究所,教育部和卫生部神经科学重点实验室,北京100191 [3]首都医科大学附属北京儿童医院耳鼻喉头颈外科,儿童耳鼻喉头颈外科疾病北京市重点实验室,北京100045

出　　处：《中国疼痛医学杂志》2016年第9期648-654,共7页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金(81500942,81371237,61527815);国家重点基础研究发展计划(“973”)项目(2013CB531905);北京市优秀人才培养资助项目(2015000021469G204);首都医科大学基础-临床科研合作项目(15JL70)

摘　　要：目的:探讨新生大鼠手术切口对脊髓小胶质细胞、BDNF含量的影响。方法:健康新生SD大鼠128只,采用随机数字表法分为假手术组(Sham组)和后足切口组(Incision组)。后足切口术前、术后分别进行脊髓小胶质细胞标记物钙离子结合蛋白1的免疫荧光染色;酶联免疫吸附测定法检测BDNF含量;测定两组痛阈。术前预先给予小胶质细胞抑制剂米诺环素,检测BDNF含量。结果:Incision组大鼠术后1、3 d钙离子结合蛋白1阳性染色增加。术后1 d、3 d、7 d、14 d脊髓背角BDNF含量上调,3 d到达顶峰,之后逐渐降至Sham组水平。术前预先给予米诺环素可缓解手术切口造成的BDNF上调。术后6 w、10 w热痛阈升高。结论:新生大鼠手术切口导致脊髓小胶质细胞激活增多,BDNF含量呈时间依赖性增加。Objective： To explore the effects of neonatal rat hind paw incision on the activation of spinal microglia and the change of BDNF expression. Methods： One hundred and twenty-eight neonatal spragueDawley rats were randomly divided into incision-operated and sham group. The alterations of microglia activation and BDNF expression in the spinal cord after operation were detected by immunofluorescence staining and enzyme-linked immunosorbent assay（ELISA） at 0d（before operation）, 1 d, 3 d, 7 d, 14 d, 21 d, 6 w, and 10 w after operation. Changes in the pain threshold after neonatal hind paw incision were tested at 14 d, 6 w and 10 w. We also detected the BDNF content of the hind paw incision rats pretreated with microglia inhibitor minocycline. Results： Compared with sham rats, the ipsilateral ionized calcium binding adaptor molecule 1（Iba1）- positive staining was significantly up-regulated on day 1 and day 3 after the hind paw incision. Moreover, the BDNF content in the spinal dorsal horn was also significantly increased in a time-dependent manner from day 1 to day 14, peaked on day 3 after neonatal hind paw incision. Pretreatment with minocycline prior to hind paw incision significantly alleviated incision induced up-regulation of the BDNF content. In addition, a prominent increase in the paw withdrawal threshold was also found at 6 w and 10 w after the hind paw incision. Conclusion： These data suggest that neonatal hind paw incision elicits the activation of microglia and up-regulates the expression of BDNF in the spinal dorsal horn.

关 键 词：脑源性神经营养因子 脊髓背角 新生大鼠 术后切口痛 

分 类 号：R722.1[医药卫生—儿科]