不同取样方法对苜蓿雄性不育系Ms-4RNA质量的影响  

EFFECT OF DIFFERENT SAMPLING METHODS ON THE EXTRACTION OF RNA FROM ALFALFA MALE STERILE LINE MS-4

作　　者：高翠萍[1] 孙川茹 石凤翎[1] 伊风艳[2] 蔡丽艳 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010011 [2]内蒙古农牧业科学院,呼和浩特010031 [3]内蒙古自治区林业检测规划院,呼和浩特010020

出　　处：《内蒙古农业大学学报（自然科学版）》2016年第4期73-76,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31302020);国家自然科学基金(31260576);教育部科技创新团队(IRT1259)

摘　　要：本文利用试剂盒法研究了冰盒保存不同时间对苜蓿雄性不育系MS-4花蕾和小花的RNA质量影响,拟筛选出适宜苜蓿雄性不育系MS-4 RNA研究的有效取样方法,解决远距离携带液氮取材困难的问题。结果表明:苜蓿雄性不育系MS-4花蕾的和小花RNA取样后冰盒保存6h内,琼脂糖凝胶电泳检测苜蓿雄性不育系MS-4总RNA,条带清晰,无拖尾现象,28s与18s的比例约为2:1,A_(260)/A_(280)的值在1.90-2.15,能保证提取的RNA的完整性,可作为苜蓿雄性不育系MS-4分子生物学研究的有效材料。因此,苜蓿雄性不育系MS-4花蕾和小花冰盒保存6h是基地取样短途运输的允许时间。In this paper, the effect of different storage time in ice box on RNA quality male sterile lines MS -4＇s buds and flowers by kit method was studied. It will select effective sampling method for RNA research of male sterile lines MS -4 and to solve the problem of the difficulty of carrying liquid nitrogen in a long distance. The results showed that RNA which storage in ice box for 6 hours has clear bands, no tail phenomenon, 28s and 18sratio around 2：1, A260 / A280 value between 1.9 -2.15, it can guarantee the integrity of RNA. The sample which storage in ice box for 6 hours can become an effective material for molecular biology study of MS - 4. Therefore, storage in ice box for less than 6 hours is suitable time for sampling of alfalfa male sterile lines MS - 4 in short dis- tance transport.

关 键 词：苜蓿雄性不育系MS-4 RNA 试剂盒法 冰盒储存 

分 类 号：Q331[生物学—遗传学]