安神方镇静催眠作用及其机制初探  

Sedative-hypnotic Effect and Its Mechanism of AnShenFang

作　　者：魏新智[1] 樊晖[1] 王清华[1] 闵冬雨[1] 王英[1] 王珲[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110847

出　　处：《中医药信息》2016年第5期48-51,共4页Information on Traditional Chinese Medicine

基　　金：2014中国博士后科学基金项目(No.2014M551121);辽宁省教育厅重点实验室基础研究项目(No.LZ2014043)

摘　　要：目的:探讨安神方镇静催眠作用及其可能机制。方法:观察安神方对小鼠自主活动的影响、对戊巴比妥钠阈剂量下小鼠入睡潜伏期及睡眠时间的影响;通过观察安神方对失眠大鼠全脑单胺类神经递质NE、DA、5-HT、5-HIAA的影响,探讨其抗失眠的可能机制。结果:与对照组比较,安神方中、高剂量组能明显减少小鼠自主活动(P<0.05);明显缩短小鼠入睡时间(P<0.01),延长小鼠睡眠时间(P<0.01);与模型组比较,安神方中、高剂量组明显升高失眠大鼠脑内5-HT含量(P<0.05)、降低失眠大鼠脑内DA(P<0.05,P<0.01)的含量。结论:本实验条件下,安神方有一定的镇静催眠作用,其机制可能与调节脑内单胺类神经递质有关。Objective：To discuss the sedative - hypnotic effect of AnShenFang and its possible mechanism. Methods ：The effect of AnShenFang was observed in the spontaneous activity of mice, the sleep latency and sleep duration of mice when they were given pentobarbital sodium in threshold dose. Observe its effect on the brain monoamine neurotransmitters such as NE, DA, 5 - HT and 5 - HIAA rats with insomnia and explore its possible mechanism. Results： The experimental results showed that the AnShenFang could inhibit the sponta- neous activity of mice（P 〈0.05）,shorten sleep latency（P 〈0.01 ） and prolong sleep time（P 〈0.01 ） in its medium and high dose groups compared with the control group. AnShenFang could significantly increase the 5 - HT content in brain of insomnia rats （ P 〈 0.05 ） and could significantly reduce the DA content in brain of insomnia rats（P 〈0.05 ,P 〈0.01 ）in its medium and high dose groups compared with the model group. Con- clusion： Under the experimental condition, AnShenFang has a sedative -hypnotic effect, and its mechanism may be related to regulating the monoamine neurotransmitters in rats＇ brain.

关 键 词：失眠 自主活动 5-HT NE DA 5-HIAA 

分 类 号：R285.5[医药卫生—中药学]