蛇葡萄素钠对肿瘤细胞及正常细胞毒性作用的比较  

Selective cytotoxic effect of ampelopsin sodium on tumor and normal cells in vitro

作　　者：翟晶[1,2] 王艳红[1,2] 高明堂[1] 吴勇杰[1] 

机构地区：[1]兰州大学基础医学院药理学研究所,兰州730000 [2]甘肃省人民医院,兰州730000

出　　处：《中药药理与临床》2016年第4期8-12,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广东省科技厅省部产学研合作项目(No 2007A090302025)

摘　　要：目的：研究蛇葡萄素钠对人肺腺癌SPC-A-1细胞、人正常胚肺MRC-5细胞的毒性作用。方法：应用MTT法检测细胞增殖,流式细胞仪检测细胞周期和细胞凋亡;透射电子显微镜（TEM）观察细胞超微结构的变化。结果：MTT法实验表明,蛇葡萄素钠、卡铂显著抑制SPC-A-1细胞的增殖,呈现浓度依赖性,IC50分别为57.18±9.42 ug/ml、33.56±11.2 ug/ml,但对MRC-5细胞增殖的抑制作用不明显,IC50分别大于5000 ug/ml及500ug/ml;流式细胞检测分析表明,25~100ug/ml的蛇葡萄素钠可使SPC-A-1细胞增殖阻滞于S期,G0/G1期细胞明显减少,且呈浓度依赖性,50、100 ug/ml的蛇葡萄素钠可使SPC-A-1细胞发生明显凋亡,而蛇葡萄素钠对MRC-5细胞周期及凋亡的影响不明显。蛇葡萄素钠浓度为50、100ug/ml时,透射电镜下可见SPC-A-1细胞表现出典型的凋亡特征,而MRC-5细胞变化不明显。结论：蛇葡萄素钠对肿瘤细胞具有较强的毒作用,对正常细胞毒性较小。Objective： To study the cytotoxic effects of the ampelopsin sodium on human lung cancer cell line SPC-A-1, human embryonic lung cellline MRC-5. Methods： Use the MTT colorimetric method to investigate cytotoxic effect; The changes of cell cycles and the ceil apoptosis were analyzed with flow cytometry（FCM）. The ultrastructural changes of ceil were observed under transmission electron microscopes （TEM）. Results： The ampelopsin sodium and carboplatin had obvious cytotoxicity on the SPC-A-1 cells in a concentration-dependent manner and it＇s IC50 value was 57.18 ±9.42 ug/ml and 33.56 ± 11.2 ug/ml, but on the MRC-5 ceils did not show obvious cytotoxicity and it＇s IC50 value was greater than 5000ug/ml and 500 ug/ml respectively. The results of cell cycle and ceil apaptosis analysis showed that hampered the prolifera- tion and induced their apaptosis of SPC-A-1 ceils in concentration-dependent manner, but not in MRC-5 cells. When the concentration of am- pelopsin sodium was 50 or 100 ug/ml, the typical apoptosis morphological changes of SPC-A-lwere observed but the changes of MRC-5 cells were few under the TEM. Conclusion： The ampelopsin sodium has stronger cytotoxicity against tumor cell lines than normal ceils.

关 键 词：蛇葡萄素钠 肿瘤细胞株 正常细胞株 细胞毒性 细胞凋亡 超微结构 

分 类 号：R285[医药卫生—中药学]