桂花根茎中一新成分的镇痛抗炎活性初步研究  

Study on the analgesic and anti-inflammation effects of a new component from the roots of Osmanthus fragrans 'rixianggui'

作　　者：李姗忆 白雪梅[1] 周奋进[1] 乔春林[1] 黄静[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《中药药理与临床》2016年第4期38-41,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究从日香桂根茎中获得的一新活性成分(GH-0521)的急性毒性、亚急性毒性、镇痛、抗炎作用。方法:按照新药临床前毒理学研究指导原则,以小鼠为对象对GH-0521进行急性毒性、亚急性毒性试验。采用醋酸扭体试验、二甲苯耳廓肿胀试验进行镇痛、抗炎活性研究。结果:急性毒性试验:12h内GH-0521总给药剂量为5g/kg的情况下,连续观察14天,小鼠生理状况良好,体重增长正常,无死亡现象;解剖后未发现肉眼任何明显异常。亚急性毒性试验:3个GH-0521给药组连续给药(0.09g/kg,0.18g/kg和0.27g/kg)2周,小鼠生理状况良好,体重增长正常,无死亡现象,各项血常规和主要器官脏器系数也均未见明显异常。镇痛试验:3个GH-0521给药组(0.06g/kg,0.12g/kg和0.24g/kg),观察小鼠对醋酸引起痛反应的扭体次数,计算各组小鼠对痛反应的抑制率分别为25%、39%和13%。二甲苯耳廓肿胀试验:3个GH-0521给药组(0.06g/kg,0.12g/kg和0.24g/kg),观察各组对二甲苯引起耳廓肿胀的肿胀度,计算肿胀抑制率分别为28%、33%和36%。结论:从日香桂根茎中获得一新成分GH-0521,该成分经初步试验证明无明显急性毒性和亚急性毒性,且具有一定的镇痛抗炎效果。Objective： To investigate the acute and the sub-acute toxicities, and the analgesic, anti-inflammatory activeies of GH-0521, an ingredi- ent from the roots of Osmanthus fragrans Pdxianggui： Methods： The methods in the Guiding Principles for New Drug Toxicity Research be- fore Clinical Application were used for the acute and the sub-acute toxicity test. And the methods of acetic acid-induced writhing response test and xylene-induced ear swelling in mice test were used for the analgesic and the anti-inflammatory activity experiments. Results： After giving a most dose （test drug, 5g/kg） once, there were no mice die, and the abnormalities of the general status and the major organs were not observed in 14 days. After a two-week treatment with the test drug at different doses （0.09g/kg, 0.18g/kg, and 0.27 g/kg）, all of indexes were in the normal range. The inhibition rates of acetic acid-induced pain response and xylene-induced ear swelling in mice by test drug were 25%, 39%, and 13%, and 28%, 33% and 36% （0.06 g/kg , 0.12g/kg , and 0.24 g/kg）, respectively. Conclusion： The above exper- iments showed there were the activities of analgesic and anti-infflammation, and no any the acute and the sub-acute toxicities of the ingredient （ GH-0521 ） from the roots of O. fragrans Rixianggui.

关 键 词：日香桂 GH-0521 急性毒性 亚急性毒性 镇痛 抗炎 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]