不同处理因素对呼吸道合胞病毒感染人支气管上皮细胞表达血管内皮生长因子的影响  

Impact of different treatment factors on expression of vascular endothelial growth factor in primary cultured human bronchial epithelial cells in-fected with respiratory virus

作　　者：高元妹[1] 徐仲[1] 黄东健[1] 蔡绍曦[2] 

机构地区：[1]广州医科大学附属第三医院重症医学科,广东广州510150 [2]南方医科大学南方医院呼吸科,广东广州510515

出　　处：《中国当代医药》2016年第27期15-18,共4页China Modern Medicine

摘　　要：目的探讨不同处理因素对呼吸道合胞病毒(RSV)感染原代培养的人支气管上皮细胞(NHBE)表达血管内皮细胞生长因子(VEGF)的影响。方法通过荧光定量PCR、ELISA检测不同处理因素对RSV感染NHBE VEGF表达的影响。结果 RSV感染NHBE后,NHBE对VEGF的表达明显增加,呈明显的时间依赖性和浓度依赖性,地塞米松可以显著抑制RSV感染NHBE细胞的VEGF表达,与RSV感染组比较,差异有统计学意义(P<0.05)。25羟基维生素D3单独作用可以刺激NHBE细胞表达VEGF,但可以抑制RSV感染NHBE的VEGF表达,与RSV组比较,差异有统计学意义(P<0.05)。结论 RSV增加了NHBE对VEGF的表达,地塞米松和25羟基维生素D3可以减弱这种效应。Objective To explore the impact of different treatment factors on expression of vascular endothelial growth factor（VEGF） in primary cultured nornal human bronchial epithelial cells（NHBE） infected with respiratory syncytial virus（RSV）.Methods The impact of different treatment factors on expression NHBE VEGF infected with RSV was tested by using fluorescence quantitative PCR and ELISA.Results After NHBE infected with RSV,the expression of NHBE VEGF was significantly increased,and with obvious time dependence and concentration dependence,Dexamethasone could sig nificantly inhibit expression of VEGF in NHBE infected with RSV,and the difference was statistically significant compared with RSV infection group（P〈0.05）.25 hydroxy vitamin D3 alone using could stimulate that NHBE expressed VEGF,but it could inhibit that NHBE infected with RSV expressed VEGF,and the difference was statistically significant compared with RSV infection group（P〈0.05）.Conclusion RSV increases the expression of VEGF in bronchial epithelium,while Dexamethasone and 25 hydroxy vitamin D3 can reduce this effect.

关 键 词：支气管哮喘 呼吸道合胞病毒 血管内皮生长因子 支气管上皮 维生素D 地塞米松 

分 类 号：R593.1[医药卫生—内科学]