清热解毒合剂质量标准研究  

Quality Standard of Qingrejiedu Heji

作　　者：王瑞 孙晓迪 任笑传 高嵩[2] 王苏会 李艳辉 李秀敏 WANG Rui SUN Xiao-di REN Xiao-chuan GAO Song WANG Su-hui LI Yan-hui LI Xiu-min(Department of Pharmacy, General Hospital of Army,Beijing 100700,China Department of Pharmaceutical Preparation,Tianjin Sanatorium of Beijing Military Command ,Tianjin 300381 ,China)

机构地区：[1]陆军总医院药剂科,北京100700 [2]北京军区天津疗养院制剂室,天津300381

出　　处：《解放军药学学报》2016年第4期322-325,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：2014年度军队医疗机构制剂标准提高科研专项课题;No.14JZJ02-1

摘　　要：目的提高清热解毒合剂的质量标准。方法以TLC法对清热解毒合剂中的金银花、甘草、知母等中药进行定性鉴别,以HPLC法测定制剂中的黄芩苷和绿原酸的含量。结果处方中金银花、甘草、知母、黄芩和连翘的薄层色谱斑点清晰,分离较好,阴性对照无干扰。黄芩苷在12.06~193.00μg·ml^(-1)范围内,峰面积与进样浓度呈良好线性关系(r=0.9949),平均加样回收率为100.33%,RSD为1.36%(n=6)。绿原酸在6.76~216.4μg·ml^(-1)范围内,峰面积与进样浓度呈良好线性关系(r=0.9999)。平均加样回收率为100.99%,RSD为1.105%(n=6)。结论该方法简便、可靠、专属性强,可作为清热解毒合剂质量控制方法。Objective To improve the quality standard of Qingrejiedu Heji. Methods Qualitative identification of Lonicera japonica Thunb.,Glycyrrhiza uralensis Fisch. and Anemarrhena asphodeloides Bge. in Qingrejiedu Heji was performed by TLC. The content of baicalin and chlorogenic acid was determined by HPLC. Results Describable characteristics of Lonicera japonica Thunb.,Glycyrrhiza uralensis Fisch.,Anemarrhena asphodeloides Bge.,Scutellaria baicalensis Georgi and Forsythia suspensa（ Thunb.） Vahl were displayed by TLC. The linear range of baicalin was 12. 06- 193 μg·ml-1（ r = 0. 9949） with an average recovery of 100. 33%（ RSD = 1. 36%,n = 6）. The linear range of chlorogenic acid was 6. 76- 216. 4 μg·ml-1（ r = 0. 9999） with an average recovery of100. 99%（ RSD = 1. 105%,n = 6）. Conclusion This method is simple,reliable and specific,which can be used for quality control of Qingrejiedu Heji.

关 键 词：清热解毒合剂 金银花 甘草 知母 黄芩苷 绿原酸 TLC HPLC 

分 类 号：R927.2[医药卫生—药学]