厚壁毛竹快速高生长期竹秆ATP酶超微细胞化学定位  

Ultracytochemical Localization of ATPase during the Rapid Elongation Growth Stage in Phyllostachys edulis 'Pachyloen' Culms

作　　者：许婷婷[1] 杨光耀[1] 杨清培[1] 于芬[1] XU Tingting YANG Guangyao YANG Qingpei YU Fen(Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Jiangxi Agricultural University, Nanchang 330045, Chin)

机构地区：[1]江西农业大学江西省竹子种质资源与利用重点实验室,南昌330045

出　　处：《西北植物学报》2016年第8期1566-1574,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31460177;31000289)

摘　　要：采用电镜细胞化学技术对厚壁毛竹(Phyllostachys edulis‘Pachyloen’)快速高生长期竹秆节间的伸长发育过程(包括:分生细胞期、伸长初期、快速伸长期和成熟期四个阶段)进行ATP酶超微细胞化学定位,以揭示竹秆节间快速伸长的细胞学基础。结果表明:分生细胞期,细胞质膜、核膜、细胞器膜系统上等均有很强的ATP酶活性。伸长初期,节间上部基本组织细胞质膜上ATP酶活性较强,且短细胞质膜上的ATP酶活性更强,节间基部各细胞均未观察到ATP酶活性。快速伸长期,节间基部基本组织ATP酶活性较节间上部高,细胞质膜、运输小泡膜、胞间隙及胞间连丝上均有ATP酶活性。成熟期,仅节间上部基本组织质膜上有较弱的ATP酶活性。ATP酶在节间伸长过程中主要参与新细胞壁物质的分泌和共质体运输,促进新细胞壁的形成,晶体和淀粉粒体外膜上ATP酶活性的存在表明其具有贮存物质的作用。节隔缺失节的节间基部未观察到ATP酶活性,节部韧皮结细胞ATP酶活性较高,节隔的缺失引起节部与节间与物质运输有关结构的变化,进而影响节间伸长生长。The ultracytochemical localization of ATPase during the rapid height growth stage in Phyllostachys edulis ‘Pachyloen＇culms was studied with cytochemical technology.The development of internode elongation can be divided into four stages：meristematic stage,initial elongation stage,rapid elongation stage and maturity stage.The results showed that the plasma membrane,karyotheca,organelle membrane system all had high ATPase activity at the meristematic stage.During the initial elongation stage,the plasma membrane of the ground tissue had high ATPase activity in the upper part of the internode,while the short parenchyma cells had higher ATPase activity.There is no ATPase deposition in any cells of the basic part of internode.During the rapid elongation stage,ATPase activity of ground tissue in the basic part of internode was higher than that in the upper part,where the plasma membrane,transport ves-icle,intercellular space and plasmodesma all had ATPase activity.Only the plasma membrane of ground tissue in the upper part of internode had low ATPase activity during the maturity stage.During the elongation growth of internode,ATPase was mainly related with synthesis and transport of new cell wall materials,while ATPase around the crystals and starch grains outer membrane played a role in storing materials.At the basic part of internode which had no diaphragm,ATPase activity was not observed,while phloem ganglion had high ATPase activity in the node.The changes of the structure related to mass transport between internode and node were caused by the loss of diaphragm,furthermore influenced internode elongation.

关 键 词：厚壁毛竹 ATP酶 高生长 竹秆 节间伸长 

分 类 号：Q944.62[生物学—植物学]