不同浓度载万古霉素半水硫酸钙/丝素微球复合人工骨材料的制备及性能研究  

Preparation and Property Investigation of Vancomycin Loaded Calcium Sulfate Hemihydrate/Silk Fibroin Microsphere Artificial Bone Composites with Different Concentration

作　　者：刘力明[1] 连小洁[1] 刘世超[1] 吴亚丽[1] 黄棣[1] 王晓君[1] 王秀梅[2] 崔福斋[2] LIU Liming LIAN Xiaojie LIU Shichao WU Yali HUANG Di WANG Xiaojun WANG Xiumei CUI Fuzhai(Institute of Mechanics, Taiyuan University of Technology, Taiyuan 030024, China Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China)

机构地区：[1]太原理工大学力学学院,太原030024 [2]清华大学材料系,北京100084

出　　处：《生物医学工程学杂志》2016年第5期903-910,共8页Journal of Biomedical Engineering

基　　金：国家自然科学基金青年科学基金资助项目(51502192);山西省基础研究计划项一般青年基金资助项目(2014021039-6;2013021014-2);山西省高等学校科技创新资助项目(2015142);太原理工大学校青年基金资助项目(2014TD066;2013Z020);太原理工大学引进人才资助项目(tyut-rc201270a);太原理工大学技术服务资助项目(143230043-J)

摘　　要：本文以丝素蛋白为载体,以万古霉素(VCM)为药物模型,采用水-油相溶解扩散方法制备了不同浓度的丝素微球(SFM),并进一步构建了载VCM半水硫酸钙(CSH)/SFM复合人工骨材料。实验中用扫描电子显微镜(SEM)观察材料的表面形态,用傅里叶变换红外吸收光谱(FTIR)研究材料的构象,用抑菌圈实验验证材料的抑菌性能,用紫外/可见分光光度计检测材料的药物释放性能,用万能试验机测定样品的抗压性能。结果表明,随着丝素浓度从20、40mg/mL增加到60mg/mL,丝素构象及抑菌性没有显著改变,而SFM粒径增加,药物释放速率减慢;同时在CSH中复合SFM后,随着SFM丝素浓度增加,复合人工骨材料药物释放速率逐渐减小,压缩断裂功逐渐增加。上述结果表明,本研究在一定程度上改进了CSH存在的脆性大、作为药物载体前期释放快的缺点,有望在临床感染性骨缺损的治疗中取得更好的应用成效。With silk fibroin and vancomycin （VCM） as carrier and drug model, respectively, we prepared silk fibroin microspheres （SFM） with different concentration using the water-in-oil emulsion solvent diffusion method. We further developed VCM loaded calcium sulfate hemihydrates （CSH）/SFM artificial bone composites. In this study, surface morphology of the materials was observed using scanning electron microscope （SEM）. Structure of the materials was studied with Fourier transform infrared spectroscopy （FTIR）. Antibacterial activity of the materials was validated with the inhibition zone test. Drug release property of materials was evaluated using ultraviolet/visible spectrophotometry. Mechanical property of the materials was tested using computer-controlled electronic universal testing machine. The results showed that silk fibroin concentration had no significant effect on molecular conformation and antibacterial property of the SFM. The average diameter of SFM increased and the release rate decreased gradually as the silk fibroin concentration increased. The release rate decreased and the compressive fracture work increased as the silk fibroin concentration increased when adding SFM to CSH. This composite had partly corrected the disadvantages of CSH including the high brittleness and initial burst release. The research would have a good application foreground in the clinical treatment of infectious bone defect.

关 键 词：丝素微球 半水硫酸钙 万古霉素 药物释放速率 力学性能 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]