两种聚乳酸改性材料的体外对比实验研究  

Experimental Comparison Research between Two Kinds of Modified Poly(lactic acid)Material In Vitro

作　　者：王玲玲[1] 崔志明[1] 徐冠华[1] 李卫东[1] 保国锋[1] 孙郁雨[1] 张金龙[1] WANG Lingling CUI Zhiming XU Guanhua LI Weidong BAO Guofeng SUN Yuyu ZHANG Jinlong(Department of Spinal Surgery, the Second Affiliated Hospital of Nantong University, Nantong 226001, China)

机构地区：[1]南通大学第二附属医院脊柱外科,南通226001

出　　处：《生物医学工程学杂志》2016年第5期911-915,共5页Journal of Biomedical Engineering

基　　金：江苏省卫生科研资助项目(Z201316);南通市应用研究计划资助项目(BK2014006)

摘　　要：为对比两种聚乳酸改性材料聚乳酸-壳聚糖(PLA-CTS)和聚乳酸-乙醇酸(PLA-PGA)的特性,本实验分别制备PLA-CTS和PLA-PGA支架,电镜下观察支架形态,计算孔隙率,测定支架降解后溶液的pH值。同时培养大鼠嗅鞘细胞(OECs),分别与两种支架混合培养,同时将单纯培养的OECs做为对照组,测定并比较两种支架上OECs的增殖力、黏附率,并在荧光镜下观察支架上OECs的生长情况。结果显示,制备的PLA-CTS和PLA-PGA支架均为多孔立体结构,PLA-CTS支架孔隙率为91%,PLA-PGA支架孔隙率为87%。随着时间延长,支架降解液的pH值逐渐下降,而PLA-PGA的下降速度要快于PLA-CTS。OECs接种到两种支架后,大多数能在支架上生长良好,MTT检测及荧光显微镜下观察细胞核数目两组差异无统计学意义,但PLA-CTS组的细胞黏附率要明显高于PLA-PGA。结论表明与PLA-PGA相比,PLA-CTS可能是做为OECs支架更佳的材料。This study aims to compare two kinds of modified poly （lactic acid） （PLA） materials：PLA-chitosan （PLA-CTS） and PLA-poly （glycolic acid） （PLA-PGA）. PLA-CTS and PLA-PGA scaffolds were prepared and observed under electron microscope. The scaffold porosity was calculated and the pH of the degradation solution was measured. Then rat olfactory ensheathing cells （OECs） were cultivated, and mixed cultured respectively with two scaffolds as two groups. The proliferation, adhesion rate and growth condition of the OECs were observed and compared between the two groups. Results showed that both the prepared PLA-CTS and PLA-PGA scaffolds were three-dimensional porous structure and the porosity of PLA-CTS was 91%, while that of PLA-PGA was 87%. The pH of degradation solution decreased gradually, of which PLA-PGA fell faster than PLA-CTS. After added to the two scaffolds, most OECs could grow well, and there were no significant differences between the two groups on MTT test and nuclei number determined by fluorescent microscope. However, the cell adhesion rate of PLA-CTS group was significantly higher than that of PLA-PGA. It can be concluded that compared with PLA-PGA, PLA-CTS might be a better choice as OECs scaffold.

关 键 词：聚乳酸 聚乙醇酸 壳聚糖 嗅鞘细胞 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] Q813.11[医药卫生—基础医学]