CD14和IL-8基因多态性与新生儿坏死性小肠结肠炎易感性的关联性分析  

Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibility of necrotizing enterocolitis

作　　者：田佳怡[1] 朱彤[1] 王健[1] 房明丽[2] 严超英[1] 

机构地区：[1]吉林大学第一医院新生儿科,吉林长春130021 [2]吉林大学第一医院儿科研究所,吉林长春130021

出　　处：《吉林大学学报（医学版）》2016年第5期958-962,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(3D513S393428)

摘　　要：目的：探讨白细胞分化抗原14（CD14）-159C/T（rs2569190）和白细胞介素8（IL-8）-251A/T（rs4073）基因多态性与坏死性小肠结肠炎（NEC）易感性之间的关系,阐明NEC易感性的可能影响因素,为NEC的发病机制研究提供遗传学理论依据。方法：选取28例NEC新生儿（NEC组）和41例非NEC新生儿（对照组）作为研究对象,提取并应用基因聚合酶链式反应（PCR）扩增其外周血DNA,并采用Sanger基因测序方法,检测CD14-159C/T和IL-8-251A/T区域的等位基因频数和基因型频数的分布,探讨其与NEC易感性的关联性。结果：CD14-159C/T和IL-8-251A/T位点基因型频数分布符合Hardy-Weinberg平衡定律（P〉0.05）;CD14-159C/T的等位基因和基因型频数分布在2组间比较差异无统计学意义（P〉0.05）;IL-8-251A/T位点的基因型频数分布在2组间比较差异无统计学意义（P〉0.05）;而NEC组IL8-251A/T基因位点T等位基因频数分布高于对照组（χ~2=4.184,P=0.041,OR=2.14,95%CI：1.03~4.46）。结论：CD14-159C/T的基因位点多态性和NEC的发病无关联,而IL-8基因的-251T位点与NEC发病易感性存在关联,IL-8基因的-251T等位基因突变可能是NEC的危险因素之一。Objective：To investigate the relationship between the gene polymorphism cluster of differentiation 14 （CD14）-159C/T （rs2569190）,and interleukin-8 （IL-8）-251A/ T （rs4073）and the susceptibility of necrotizing enterocolitis （NEC）,to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods：Total 28 newborns with NEC and 41 newborns without NEC were selected.The amplification of peripheral blood DNA was conducted by PCR.The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied.Results：The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium （P 〉0.05）.There＆amp;nbsp;were no significant differences of the allelic and genotypic frequencies of CD14-159C/T,or genotypic frequencies of IL-8-251A/T between two groups （P 〉0.05）.While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group （χ2 = 4.184, P = 0.041, OR = 2.14, 95% CI： 1.03 - 4.46 ）. Conclusion：The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC,but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC.So T allele in IL-8-251A/T may be one of the danger factors of NEC.

关 键 词：坏死性小肠结肠炎 白细胞分化抗原14 白细胞介素-8 单核苷酸多态性 

分 类 号：R722.19[医药卫生—儿科]