人胃癌裸鼠原位移植瘤模型的建立及其活体荧光成像检测  

Establishment of an endogastric xenograft tumor modal in nude mice and its detection by in vivo fluorescence imaging system

作　　者：曹钟元 赵振凯[1] 相庚 倪清蓉 才延辉[2] 席宇菁 赵晶[3] 阎博[3] CAO Zhongyuan ZHAO Zhenkai XIANG Geng NI Qingrong CAI Yanhui XI Yujing ZHAO Jing YAN Bo(a. School of Student Brigad b. Deparment of Anesthesiology, Xijing Hospita e. Department of Biochemistry and Molecular Biology, the Fourth Military Medical University, Xi＇an 710032, Shaanxi, China)

机构地区：[1]第四军医大学学员旅,陕西西安710032 [2]第四军医大学西京医院麻醉科,陕西西安710032 [3]第四军医大学生物化学与分子生物学教研室,陕西西安710032

出　　处：《中国肿瘤生物治疗杂志》2016年第5期703-707,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.81372459)~~

摘　　要：目的:建立可动态实时监测的人胃癌裸鼠原位移植瘤模型。方法:将稳定表达荧光素酶的人胃癌细胞SGC-7901^(fLuc+)注入裸鼠后肢根部皮下形成皮下移植瘤。待皮下移植瘤瘤块长至直径0.5 cm时,剥取肿瘤组织,应用叠合法将瘤块原位移植于裸鼠胃小弯处,形成原位移植瘤模型。利用小动物活体荧光成像系统,每4日监测移植瘤的进展情况。荷瘤3周后,解剖荧光成像阳性小鼠,利用H-E染色、光镜下观察移植瘤的形态。结果:应用小动物活体荧光成像系统,可在荷瘤裸鼠胃部检测到逐渐增强的荧光信号。荷瘤裸鼠胃部存在肿瘤包块贴附于胃壁,包块直径为0.5~1.0 cm,包块周围与相邻组织器官边界清晰,未见粘连,检查腹腔未见转移,未见腹水形成。应用叠合法构建人胃癌裸鼠原位移植瘤模型成功率为95%(19/20)。对荷瘤胃组织H-E染色后,可见异常肿瘤细胞,肿瘤包膜完整,符合胃癌组织学特征。结论:成功建立了操作简便、成瘤率高的可动态监测的人胃癌裸鼠原位移植瘤模型,为研究胃癌发生机制、研发抗癌药物提供了理想的实验工具。Objective：To establish an endogastric xenograft tumor model in nude mice which can be dynamically monitored by in vivo fluorescence imaging system. Methods： The SGC-7901fLuc＋ cell line stably expressing luciferase was injected subcutaneously into nude mice to develop transplanted tumor. Subcutaneously transplanted tumor （with diameter of 0.5 cm） was collected and orthotopically overlapping sutured into lesser gastric curvature of nude mice. Tumor growth in rats was monitored using in vivo fluorescence imaging system every 4 days. At 3 weeks post-tumor implantation, the fluorescence-positive mice were sacrificed and H-E staining was performed to observe the morphology of endogastric xenograft tumor. Results：Using in vivo fluorescence imaging system, gradually enhanced fluorescence signal could be detected on the gastric area of tumor bearing nude mice. Tumor block grew locally around the lesser gastric curvature, with typical gastric carcinoma morphology as assessed by H-E staining. The diameter of the tumor block was 0.5-1 cm. The boundary of tumor and adjacent tissue was clear. No adhesion, no metastasis was found in the abdominal cavity, and no formation of ascites was found. The successful rate of endogastric xenograft tumor model of human gastric carcinoma in nude mice was 95% （19/20）. Conclusion： The endogastric xenograft tumor model in nude mice was successfully established. This model provided an ideal experimental tool for studying the mechanism of human gastric carcinoma and the development of anticancer drugs.

关 键 词：胃原位移植瘤模型 活体荧光成像技术 SGC-7901细胞株 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]