TiO_2纳米管阵列孔径调控葡萄糖氧化酶生物传感器性能  

Regulate properties of glucose oxidase biosensors through pore sizes of TiO_2 nanotube arrays

作　　者：饶超[1] 董依慧 庄伟[2] 邬新兵[1] 洪启亮 刘畅[1] 陆小华[1] 

机构地区：[1]南京工业大学材料化学工程国家重点实验室,江苏南京210009 [2]南京工业大学国家生化工程技术研究中心,江苏南京211816

出　　处：《化工学报》2016年第10期4324-4333,共10页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2013CB733501);国家自然科学基金项目(21136004;21476106;21506090);江苏省自然科学基金项目(BK20130929);江苏高校优势学科建设工程项目~~

摘　　要：采用电化学阳极氧化法制备出不同孔径(21、62、83、102 nm)的Ti O_2纳米管阵列(TNA),研究了孔径对固定化葡萄糖氧化酶(GOx)的传感器性能的影响。循环伏安测试结果表明固定在不同孔径大小的TNA上的GOx在葡萄糖溶液中均具有良好的酶活性。计时电流法和交流阻抗法测试发现,当孔径是83 nm时,灵敏度达到最大值27.2μA·(mmol·L-1)-1·cm-2。调控TNA的孔径可改变固定化GOx的活性及溶液扩散阻抗,从而显著提高生物传感器性能。Highly ordered Ti O_2 nanotube arrays（TNA） with controlled pore sizes in a series of 21, 62, 83 and 102 nm were synthesized using constant current oxidization method. Glucose oxidases（GOx） were immobilized on TNA by physical adsorption, and the GOx activities on TNA were investigated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. All immobilized GOx had good oxidizing activities in glucose solution and GOx on 83 nm-pore-sized TNA showed the best sensibility of 27.2 μA·（mmol·L-1）-1·cm-2, which was probably due to a combination effect of reduced diffusion resistance and small Michaelis constant of the immobilized GOx. The experimental results had demonstrated that controlling pore sizes of TNA could effectively tune sensitivity of glucose biosensors.

关 键 词：葡萄糖氧化酶 TIO2纳米管阵列 孔径 扩散阻抗 电化学 生物传感器 制备 生物过程 

分 类 号：O647[理学—物理化学]