盐胁迫下巴西蕉幼苗部分细胞膜系统Ca^(2+)-ATPase的活性变化  

Changes of Ca^(2+)-ATPase activities of part of cell membrane system in Brazilian banana seedling under salt stress

作　　者：王文昌[1,2] 乔飞[2] 江雪飞[1] 周双云[3] 吉福桑 李新国[1] 

机构地区：[1]海南大学园艺园林学院,海口570228 [2]中国热带农业科学院热带作物品种资源所,海南儋州571737 [3]广西农业科学院园艺研究所,南宁530007

出　　处：《植物生理学报》2016年第8期1199-1206,共8页Plant Physiology Journal

基　　金：国家自然科学基金(31260462);海南大学中西部计划学科建设项目(ZXBJH-XK008)~~

摘　　要：为研究盐胁迫下微粒体膜Ca^(2+)-ATPase活性变化,试验采用营养液培养的方法,研究巴西蕉幼苗在60 mmol·L^(-1)的NaCl胁迫4、8、12、24和48 h下,增加外源CaCl_2和钙离子螯合剂EGTA处理,测定其叶片和根部分细胞膜上的Ca^(2+)-ATPase活性。结果表明,巴西蕉幼苗根和叶片的质膜、液泡膜及内质网膜的Ca^(2+)-ATPase活性各不同,且CaCl_2处理的Ca^(2+)-ATPase活性均显著高于其它3种处理。在NaCl胁迫4 h时,CaCl_2处理的Ca^(2+)-ATPase活性均显著升高,而EGTA处理的则显著下降。幼苗质膜和液泡膜的Ca^(2+)-ATPase活性远远大于内质网膜Ca^(2+)-ATPase。这表明了质膜和液泡膜Ca^(2+)-ATPase活性主要参与巴西蕉幼苗Ca^(2+)稳态的调控。To study the changes in Ca2＋-ATPase activities of microsome membrane under salt stress, the Brazilian banana seedlings were treated with the exogenous CaCl2 and Ca2＋ chelating agent EGTA under 60 mmol·L-1 NaCl for 4, 8, 12, 24 and 48 h, and Ca2＋-ATPase activities of part of cell membrane in leaves and roots were determined. The results showed that Ca2＋-ATPase activities of plasma membrane, tonoplast membrane and endoplasmic reticulum membrane in roots and leaves were different, and the Ca2＋-ATPase activity with CaCl2 treatment was significantly higher than those of other three treatment. Under NaCl stress for 4 h, the Ca2＋-ATPase activities with CaCl2 treatment were significantly increased, while the Ca2＋-ATPase activities with EGTA treatment decreased significantly. The Ca2＋-ATPase activities of the plasma membrane and tonoplast membrane were much larger than that of the endoplasmic reticulum membrane. These indicated that Ca2＋-ATPase activity of plasma membrane and tonoplast membrane mainly involved in the regulation of Ca2＋ homeostasis in Brazilian banana seedlings.

关 键 词：巴西蕉幼苗 盐胁迫 Ca2+-ATPase活性 

分 类 号：S668.1[农业科学—果树学]