米氏凯伦藻多糖的酶法制备及其体外抑制肿瘤血管生成活性  

Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity

作　　者：陈宇婷[1] 孟繁桐 孔亮[1] 田夕凡 谭成玉[1] CHEN Yu-ting MENG Fan-tong KONG Liang TIAN Xi-fan TAN Cheng-yu(College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China Tianjin Deheng Science and Technology Co. ,Ltd, Tianjin 300384, China)

机构地区：[1]大连海洋大学海洋科技与环境学院,大连116023 [2]天津德恒科技有限公司,天津300384

出　　处：《天然产物研究与开发》2016年第9期1470-1474,1442,共6页Natural Product Research and Development

基　　金：国家自然科学基金(41306137);辽宁省自然科学基金(2013020127);辽宁省高校杰出青年学者成长计划(LJQ2014078)

摘　　要：研究碱性蛋白酶酶解制备米氏凯伦藻多糖及其抑制肿瘤血管活性。采用闪式萃取结合酶法制备米氏凯伦藻多糖,其适宜提取条件为碱性蛋白酶酶解温度40℃、酶解p H为8.5、酶用量为2.5%、酶解时间为2 h。其提取液经三氯乙酸除蛋白、乙醇沉淀获得米氏凯伦藻多糖。制备的米氏凯伦藻多糖在一定浓度时可抑制肿瘤细胞培养液诱导的内皮细胞增殖和迁移作用,具有一定的抑制肿瘤血管生成活性。To investigate the preparation method on karenia polysaccharides with alkali protease enzymolysis from Karenia mikimotoi and its anti-angiogenic activity. The extraction of karenia polysaccharides was obtained by flash extraction combined with enzymolysis method,and the optimal process conditions were determined as enzymolysis temperature of 40℃,p H 8. 5,enzymolysis dosage of 2. 5% and enzymolysis time of 2 h. The karenia polysaccharides were obtained by using trichloroacetic acid to get rid of protein and ethanol for precipitation. The karenia polysaccharides showed some inhibition on the viability and migration of induced human umbilical vein endothelial cells at certain concentration.

关 键 词：米氏凯伦藻 多糖 闪式萃取结合酶法 抑制血管生成 

分 类 号：O629.1[理学—有机化学]