快速检测鸡白痢沙门氏菌4种免疫传感器的对比研究  

Comparison of four kinds of immunosensor for rapid detection of Salmonella pullorum

作　　者：马骏爽 王丹 窦文超 陈妍 赵广英 

机构地区：[1]浙江工商大学食品与生物工程学院/浙江省食品安全重点实验室,浙江杭州310018

出　　处：《中国预防兽医学报》2016年第9期719-724,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目(30571623);食品科学与工程浙江省重中之重一级学科(JYTSP20141062);浙江省公共创新平台分析测试项目(2015C37023);浙江工商大学研究生科研创新基金项目

摘　　要：为改善电化学免疫传感器因电极修饰均一性和牢固性难以掌控等导致重现性和准确性较差的问题,本研究以鸡白痢沙门氏菌(S.pullorum)为检测对象,选用丝网印刷电极(SPCE)为支持电极;通过电沉积方法修饰不同材料,包括氧化石墨烯(GO)、纳米金(AuNPs)、离子液体(ILs),按不同组合修饰电极;进而结合酶标抗体制成快速检测致病菌的4种免疫电极;采用循环伏安法(CV)检测电化学响应信号;分别进行电极均一性、牢固性、灵敏度、特异性和稳定性的对比试验,综合检测结果进行择优筛选。结果表明,AuNPs和ILs对免疫传感器的各项性能特别是重现性和准确性的改善作用明显,与其它组比较差异显著,本研究可成为开发快速检测其它致病菌免疫传染器的研究模型。In order to improve the uniformity and degree of rigidity of electrochemical immunosensor, which lead to poor repeatability and accuracy, we developed four different methods for modification of immunosensor for pathogenic bacteria detection. Screen printing electrode （SPCE） modified by different combinations of materials include Graphene Oxide （GO）, Nanogold Particles （AuNPs） and Ionic Liquids （ILs） by electrodeposition method was choosen as supporting electrode, and then such modified electrode was incubated with enzyme labelled antibody to form four kinds of immunoelectrode in pathogenic bacteria detection. Electrochemical signal was detected with cyclic voltammetry（CV）, Electrode homogeneity, immobility, sensitivity, specificity and stability of the four kinds of modified electrode were compared in the experiment. The results shown that ILs and AuNPs obviously improved the performance of immunosensor, especially the repeatability and accuracy compared with other groups. In this study, S.pullorum was used as the detection subject, and it can be used as a model for the development of rapid detection of other pathogenic bacteria.

关 键 词：电化学免疫传感器 鸡白痢沙门氏菌 氧化石墨烯 纳米金 离子液体 

分 类 号：S852.61[农业科学—基础兽医学]