健脾解毒方对裸鼠皮下移植瘤Bax、Bcl-2 mRNA表达的影响  

Effects of Jianpi Jiedu Decoction on the mRNA expression of Bax and Bcl-2 in subcutaneous transplanted tumor of nude mice

作　　者：李敬瑜 万光升[1] 李萍[1] 孙珏[1] 

机构地区：[1]上海中医药大学附属普陀医院中医肿瘤科,上海200062

出　　处：《上海中医药杂志》2016年第10期77-80,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局"十二五"重点专科建设项目[国中医药医政发(2012)2号];国家中医药管理局中医药重点学科建设项目[国中医药人教发(2012)32号];上海中医药大学预算内科研项目(2013JW63)

摘　　要：目的观察中药复方健脾解毒方对裸鼠皮下移植瘤Bax及Bcl-2 mRNA表达的影响。方法采用HCT8/V细胞制作裸鼠皮下移植瘤,将32只造模成功的裸鼠随机分为4组,每组8只。空白组予0.9%Na Cl溶液腹腔注射,化疗组予长春新碱腹腔注射,中药组予健脾解毒方灌胃,联合组予长春新碱腹腔注射和健脾解毒方灌胃。测量各组皮下移植瘤的瘤重及抑瘤率,光镜观察肿瘤组织病理情况,并采用Real-time PCR法测定Bax、Bcl-2 mRNA的表达。结果与空白组比较,化疗组、中药组、联合组瘤重明显降低,差异有统计学意义(P<0.01);与化疗组比较,中药组、联合组瘤重均降低,差异有统计学意义(P<0.01);相对于空白组,化疗组、中药组、联合组瘤体抑制率分别为22.84%、38.44%、51.53%,以联合组抑瘤效果最佳。与空白组比较,化疗组、中药组、联合组Bax mRNA表达水平显著升高(P<0.05,P<0.01),Bcl-2 mRNA表达水平均显著降低(P<0.01);与化疗组比较,中药组、联合组Bax mRNA相对表达量高于化疗组(P<0.05,P<0.01),中药组、联合组Bcl-2 mRNA相对表达量均低于化疗组(P<0.01)。结论健脾解毒方通过上调Bax mRNA表达,下调Bcl-2 mRNA表达,促进肠癌细胞的凋亡,且与化疗药具有协同作用。Objective To observe the effect of Jianpi Jiedu Decoction on the mRNA expression of Bax and Bcl-2 in subcutaneous transplanted tumor of nude mice. Methods HCT8/V cells were subcutaneously injected into the nude mice to establish model. Then 32 successful modeling mice were randomly divided into four groups and 8 in each group. The blank group was treated with intraperitoneal injection of 0. 9% NaCl, the chemotherapy group was treated with intraperitoneal injection of vincristine, the Chinese medicine group was given Jianpi Jiedu Decoction by means of intragastric administration, the combination group was given vincristine with intraperitoneal injection and Jianpi Jiedu decoction with intragastric administration. The tumor weight and inhibition rate of subcutaneous transplanted tumor in each group were measured, the pathological changes of tumor tissues were observed by light microscope, and the mRNA expression of Bax and Bcl-2 were determined by Real-time PCR. Results Compared with the blank group, the tumor weight of the chemotherapy group, Chinese medicine group and combination group was significantly reduced, with statistically significant difference （P 〈 0.01 ） ; compared with the chemotherapy group, the tumor weight of the Chinese medicine group and combination group was significantly reduced, with statistically significant difference（ P 〈 0.01 ） ; compared with the blank group, the inhibition rate of tumor in the chemotherapy group, Chinese medicine group and combination group were 22.84%, 38.44%, 51.53% respectively, and the effect of the combination group was best. Compared with the blank group, the Bax mRNA expression of the chemotherapy group, Chinese medicine group and combination group was increased significantly （P 〈0.05, P 〈0.01 ） and the Bcl-2 mRNA expression was significantly decreased （P 〈0.01 ） ; compared with the chemotherapy group, the relative expression level of Bax mRNA in the Chinese medicine group and the combination group was higher than that in the chemotherapy

关 键 词：大肠癌 健脾解毒方 BAX BCL-2 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]