25羟维生素D3与抗菌肽在反复上呼吸道感染中的作用研究及相关性分析  

The role and correlation analysis between 25-hydroxy vitamin D3 and human cathelicidin in recurrent upper respiratory infection

作　　者：罗光燕 杨小琼 张芸 王孝芸 袁谢芳 王星 李国平 

机构地区：[1]西南医科大学卫生科,四川泸州646000 [2]西南医科大学附属医院炎症与变态反应实验室,四川泸州646000 [3]西南医科大学附属医院呼吸内科,四川泸州646000

出　　处：《重庆医学》2016年第29期4053-4055,4058,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81170032)

摘　　要：目的探讨25羟维生素D3与抗菌肽在反复上呼吸道感染的作用。方法选择连续3年就诊的上呼吸道感染患者,其中,1年上呼吸道感染次数大于或等于2次患者46例,男20例,女26例,平均(32.35±9.10)岁;选择15例人作为对照组,平均(31.35±10.20)岁,对照组在年龄上与上呼吸道感染患者差异无统计学意义(P>0.05)。采用酶联免疫吸附测定(ELISA)检测外周血血清中25羟维生素D3水平和诱导痰上清液抗菌肽LL-37水平并做相关性分析。用人气道上皮细胞株分析25羟维生素D3对抗菌肽LL-37水平影响。结果反复上呼吸道感染患者外周血血清25羟维生素D3水平低于对照组(P<0.05),并且反复上呼吸道感染患者诱导痰上清液人抗菌肽LL-37水平低于对照组(P<0.05)。上呼吸道感染平均次数与上呼吸道感染患者外周血25羟维生素D3呈负相关(r=-0.54,P<0.05),上呼吸道感染平均次数与诱导痰上清液抗菌肽LL-37水平呈负相关(r=-0.65,P<0.05),上呼吸道感染患者外周血25羟维生素D3和诱导谈上清液抗菌肽LL-37水平呈正相关(r=0.59,P<0.05)。体外细胞实验显示1,25羟维生素D3能上调上皮细胞抗菌肽LL-37表达。结论 25羟维生素D3的缺乏可能是上呼吸道反复感染患抗菌肽LL-37的表达下调的重要原因。Objective 25-hydroxy vitamin D3 as a key innate immune signaling,which can promote the expression of antimi- crobial peptide LL-37. This study was to explore the role of 25-hydroxy vitamin D3 and LL-37 in the recurrent upper respiratory tract infection. Methods The patients with upper respiratory tract infection in our department for three consecutive years were se- lected. The prevalence frequencies of 46 patients with upper respiratory tract infection were greater than or equal to two times one year （20 male patients and 26 female patients）. The average age was （32.35±9.10）years old. 15 cases of healthy people were re- gards as a control group,and the average age was （31.35 ± 10.20）years old. The age was no significant difference between the pa- tient with upper respiratory tract infection and control group（P〈0.05）. The level of 25-hydroxy vitamin D3 and human antimicro- bial peptide LL-37 in peripheral blood serum and supernatant of induced sputum were detected with ELISA. The correlation analysis was done with SPSS software. The effect of 25 hydroxy vitamin D3 on the level of LL-37 in the antimicrobial peptide were analyzed by human airway epithelial cell line. Results The levels of 25-hydroxy vitamin D3 from peripheral blood serum in patients with up- per respiratory tract infection were significantly lower than the healthy control group（P〈0.05）. The levels of LL-37 in patients with upper respiratory tract infection were significantly decreased,compared with healthy control group（P〈0.05）. The prevalence frequencies of upper respiratory tract infection were negatively correlated with the levels of 25-hydroxy vitamin D3（r=, 0.54, P〈 0.05） ,and prevalence frequencies of upper respiratory tract infection were also negatively associated with levels of antimicrobial peptide LL-37（r=- 0.65, P〈0.05）. The levels of 25-hydroxy vitamin D3 were positively correlated with levels of antimicrobial peptide LL-37（r=0.59 ,P〈0.05）. In vitro cell experiments showed that 25-hydro

关 键 词：反复上呼吸道感染 25羟维生素D3 抗菌肽LL-37 

分 类 号：R56[医药卫生—呼吸系统]