内质网应激相关蛋白与CXCL12蛋白在特发性肺纤维化中的表达及意义  

Expression and significance of endoplasmic reticulum stress associated protein and CXCL12 protein in the pathogenesis of idiopathic pulmonary fibrosis

作　　者：金笛[1] 何丽[2] 张海锋[3] 

机构地区：[1]湖北中医药高等专科学校内科教研室,湖北荆州434020 [2]湖北省荆州市中心医院呼吸内科,434020 [3]湖北省荆州市第二人民医院呼吸内科,434000

出　　处：《重庆医学》2016年第29期4068-4070,共3页Chongqing medicine

摘　　要：目的探讨内质网应激相关蛋白及CXCL12在特发性肺纤维化中的表达及意义。方法收集荆州市中心医院2004~2014年诊断为特发性肺纤维化且存有活检或手术标本的患者共18例,并收集正常肺组织20例作为对照,通过蛋白免疫印迹法(Western blot)检测内质网应激相关蛋白(GRP78、CHOP)及CXCL12蛋白(SDF-1)的表达,通过逆转录PCR(RT-PCR)检测肺组织中GRP78mRNA及CXCL12mRNA的表达。结果与对照相比,肺纤维化患者肺泡上皮中GRP78、CHOP、CXCL12蛋白表达均上调,且GRP78mRNA及CXCL12mRNA也表达上调。结论内质网应激相关蛋白及CXCL12在特发性肺纤维化的发病过程中起重要作用,可能参与了特发性肺纤维化的发生与发展。Objective To investigate the expression and role of endoplasmic reticulum stress associated protein and CXCL12 protein in the pathogenesis of idiopathic pulmonary fibrosis（IPF）. Methods From 2004 to 2014,1ung tissues from idiopathic pul- monary fibrosis patients（n= 18） and normal persons（n=20,control） of Jingzhou Central Hospital, were used for detecting the ex- pression of GRP78,CHOP and CXCL12 protein by Western blot,and used for detecting the expression of GRP78 mRNA and CX- CI.12 mRNA by RT-PCR. Results The expression of GRP78 ,CHOP and CXCL12 protein was up-regulated in idiopathic pulmona- ry fibrosis patients,also the expression of GRPT8 mRNA and CXCL12 mRNA were higher in patients than normal tissue. Conclu- sion Endoplasmic reticulum stress related proteins and CXCL12 play an important role in the pathogenesis of idiopathic pulmonary fibrosis, which may be involved in the occurrence and development of idiopathic pulmonary fibrosis.

关 键 词：特发性肺纤维化 内质网应激 GRP78 CHOP CXCL12 

分 类 号：R563.9[医药卫生—呼吸系统]