白藜芦醇对卵泡颗粒细胞线粒体的调节作用及人工辅助生殖效果的影响  

Effect of resveratrol on the granulosa cell mitochondrial function and ART pregnancy outcome

作　　者：陈兰[1] 胡沙[2] 李素萍[1] 

机构地区：[1]湖北民族学院附属民大医院妇科,湖北恩施445000 [2]华中科技大学同济医学院附属协和医院,武汉430022

出　　处：《重庆医学》2016年第29期4080-4082,共3页Chongqing medicine

摘　　要：目的探讨口服白藜芦醇对卵泡颗粒细胞线粒体功能及人工辅助生殖技术(ART)效果的影响。方法研究入组受试对象109例,其中,对照组59例,白藜芦醇组50例。分析其颗粒细胞氧化应激水平和线粒体功能,检测胚胎发育和妊娠结局相关指标及不良反应。结果白藜芦醇使颗粒细胞的还原型辅酶Ⅱ(NADPH)氧化活性下降了28.57%(P<0.05),同时也降低了丙二醛(MDA)水平(0.653±0.084)nmol/mL vs.(0.382±0.052)nmol/mL,(P<0.05)。白藜芦醇增加了线粒体DNA(mtDNA)拷贝数(4.62±0.24)×105 vs.(5.77±0.38)×105,(P<0.05)和ATP生成(2.516±0.160)pmol vs.(4.484±0.180)pmol,(P<0.05)。白藜芦醇组较之对照组其卵成熟率(87.75%vs.94.15%,P<0.05)、受精率(86.69%vs.91.49%,P<0.05)和优质胚胎率(44.30%vs.51.22%,P<0.05)均显著提高,且尚未发现有明显的不良反应。结论白藜芦醇可能对提高ART的成功率具有一定的辅助作用。Objective To explore the effect of resvertrol supplement to the granulosa cell mitochondrial function and assisted reproductive technology （ART） outcome. Methods The study would analyze the level of oxidative stress and mitochondrial func- tion in granulosa cell from 109 cases with or without resvertrol supplement,including 50 cases in resvertrol group and 59 cases as control group. The embryo quality, IVF-ET outcome and adverse reactions would be observed. Results Resveratrol reduced the NADPH oxidation activity of granule cells by 28.57% （P〈0.05）, and also decreased the MDA content （0. 653± 0. 084）nmol/mL vs. （0. 382± 0. 052）nmol/mL, （P〈 0. 05）. Moreover, resvertrol supplement improved the mitochondrial DNA copy number （4.62±0.24）×105 vs. （5.77±0.38）×105,（P〈0.05） and ATP production （2.516±0.160）pmol vs. （4. 484±0.180）pmol, （P〈0.05） in granulosa cell. Compared with the control group, the oocyte maturation rate （87.75% vs. 94.15 %,P〈0.05）, fertili- zation rate （86.69% vs. 91. 49%,P〈0. 05） and high quality embryo rate （44.30% vs. 51. 22%,P〈0. 05） in the resveratrol group were significantly increased. Meanwhile,the resvertrol supplement did not show any adverse effect. Conclusion Resvertrol might be a adjunctive therapy to the ART.

关 键 词：人工辅助生殖技术 白藜芦醇 颗粒细胞 

分 类 号：R714.8[医药卫生—妇产科学]