美洲大蠊提取液对大鼠难愈合创面VEGF表达影响的研究  

On Effect of American Periplaneta Extract on VEGF's Gene Expression of Rats' Chronic Refractory Wound

作　　者：曾娟妮[1] 刘筱[2] 伍伟明[2] 尹婷[2] 吴志荣[2] 李文华[2] 

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410000 [2]湖南中医药大学,湖南长沙410208

出　　处：《中国民族民间医药》2016年第19期64-66,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：湖南省中医药科研计划项目(编号:201466)支助;湖南省教育厅重点学科中医外科学一肛肠方向专项支助

摘　　要：目的:探讨外用单味动物药促进创面愈合的机制。方法:将50只大鼠分为急性创面组、模型组、贝复济组、美洲大蠊提取液组,用RT-PCR实验方法,观察大鼠皮肤修复组织VEGFmRNA的表达。结果:VEGFmRNA的表达在第5、7、11天,急性愈合创面组VEGFmRNA的表达量显著高于美洲大蠊提取液组、贝复济组及模型组(P<0.01);在第5、7、11天,美洲大蠊提取液组、贝复济组VEGFmRNA的表达量显著高于模型组(P<0.01);在第5、11天,美洲大蠊提取液组与贝复济组VEGFmRNA的表达量有统计学差异(P<0.05);第7天,肉芽组织生长期,美洲大蠊提取液组与贝复济组VEGFmRNA的表达量无统计学差异(P>0.05)。结论:美洲大蠊提取液通过提高VEGFmRNA的表达来促进创面愈合。Objective to study the external use of traditional Chinese medicine of single animal to promote the mechanism of wound healing. Methods dividing 50 rats into acute wound group, model group, rb - bfgf group, and American periplaneta extract group, using RT - PCR testing method to observe the rat＇ s expression of skin repairing tissue of VEGFmRNA. Results the expression of VEGFmRNA on the fifth day, the seventh day and the eleventh day, the acute healing wound group has a significantly higher a- mount of expression than American periplaneta extract group and rb - bfgf group （ P 〈 0.01 ） ; On the fifth day, the seventh day and the eleventh day, the expressions of VEGFmRNA of both American periplaneta extract group and rb - bfgf group are significantly higher than model group （ P 〈 0.01 ） ; On the fifth day and the eleventh day, American periplaneta extract group has difference with the rb - bfgf group on the expression quantity of VEGFmRNA （ P 〈 O. 05 ） , on the seventh day, which is the growing period of granulation tis- sue, American periplaneta extract group has no difference with rb - bfgf group on the expression quantity of VEGFmRNA （ P 〉 0. 05 ）. Conclusion American periplaneta extract promotes the wound healing by increasing the expression of VEGFmRNA.

关 键 词：美洲大蠊提取液 慢性难愈合创面 RT-PCR(逆转录聚合酶链式反应) 基因表达 

分 类 号：R285.5[医药卫生—中药学]