冷沉淀在小鼠烧伤创面铜绿假单胞菌感染模型的应用研究  

Cryoprecipitate used for the Pseudomonas aeruginosa infection in burn wound of rat model

作　　者：康琼英[1] 沈莉[2] 张庆富[3] 马西霞[1] 白雪梅[1] 温洁新[1] 

机构地区：[1]河北医科大学第一医院输血科,河北石家庄050031 [2]河北医科大学第一医院检验科,河北石家庄050031 [3]河北医科大学第一医院烧伤整形科,河北石家庄050031

出　　处：《中华医院感染学杂志》2016年第19期4345-4347,共3页Chinese Journal of Nosocomiology

基　　金：河北省卫生厅医学科技研究基金资助项目(20110293)

摘　　要：目的建立小鼠烧伤创面铜绿假单胞菌感染模型,探讨冷沉淀联合磺胺嘧啶银对烧伤创面感染的治疗作用,为临床治疗烧伤创面铜绿假单胞菌感染提供理论依据。方法建立小鼠烧伤创面铜绿假单胞菌感染模型40份,将其随机分为治疗组和对照组,均常规清创、消毒后,治疗组创面均匀涂以冷沉淀,外敷磺胺嘧啶银油纱布,对照组仅外敷磺胺嘧啶银油纱布,建模后24h观察创面菌落情况,并统计小鼠7d生存率,ELISA方法检测各时间点炎性因子IL-4、IL-10、TNF-α表达水平。结果两组小鼠模型建立成功,均检测到铜绿假单胞菌的荧光强度,治疗组小鼠菌落平荧光强度(2.72±0.68)明显低于对照组(4.04±1.02),差异有统计学意义(P<0.01);治疗组创面组织菌落计数(2.79±0.75)×106 CFU/ml,明显低于对照组(1.84±0.93)×109 CFU/ml,差异有统计学意义(P<0.05);建模7d治疗组小鼠累计生存率为90.00%明显高于对照组60.00%,差异有统计学意义(P<0.05);小鼠炎性因子IL-4、IL-10、TNF-α表达水平治疗组明显低于对照组,差异有统计学意义(P<0.05),治疗组各因子表达的时间曲线更趋于平稳。结论冷沉淀制剂对改善烧伤创面感染的作用显著,且能够明显控制铜绿假单胞菌的感染。OBJECTIVE To establish rat burn wound model with Pseudomonas aeruginosainfection and investigate the effects of cryoprecipitate combined with sulfadiazine silver to burn wound,in order to provide theory evidence for clinical treatment on P.aeruginosainfection in burn wound.METHODS A total of 40 models of rat burn wound model with P.aeruginosainfection were established,which were divided into treatment group and control group.After the routine debridement and disinfect,treatment group received cryoprecipitate coating on the wound combined oil gauze with sulfadiazine silver,but control group only received oil gauze with sulfadiazine silver.After 24 h of model establishment,colony of bacteria were under observation,and 7dsurvival rate of rat were counted.Inflammatory factors level of IL-4,IL-10,TNF-αin different time points were detected by ELISA method.RESULTS The rat models were established successfully and the fluorescence intensity of P.aeruginosa were found.The fluorescence intensity of bacteria colony in treatment group（2.72±0.68）was significantly lower than that of control group（4.04±1.02）（P〈0.01）.The count of bacteria colony in treatment group（2.79±0.75）×10^6 CFU/ml was significantly lower than that in control group（1.84±0.93）×10^9 CFU/ml（P〈0.05）.After 7dof model established,survival rate of treatment group（90.00%）was significantly higher than that of control group（60.00%）（P〈0.05）.The levels of IL-4,IL-10 and TNF-αof treatment group were significantly lower than those of control group（P〈0.05）.The time curve of all factors in treatment group tended to be steady.CONCLUSIONThe effect of cryoprecipitate is significant in improving infection of burn wound,and it could obviously control the infection of P.aeruginosa.

关 键 词：冷沉淀 烧伤 铜绿假单胞菌 感染 

分 类 号：R378.991[医药卫生—病原生物学]