感染性角膜炎患者的病原菌分布与耐药性分析  

Pathogen distribution and drug resistance of infectious keratitis

作　　者：杨杰[1] 汪怿[1] 温积权[1] 季娴[1] 刘健[1] 江媛[1] 

机构地区：[1]浙江医院眼科,浙江杭州310013

出　　处：《中华医院感染学杂志》2016年第19期4497-4499,共3页Chinese Journal of Nosocomiology

基　　金：浙江省卫生厅医药卫生计划基金项目(2013KYA132)

摘　　要：目的研究感染性角膜炎患者的病原菌分布及其耐药性,为临床治疗提供依据。方法选取2012年12月-2015年12月医院诊治87例感染性角膜炎患者资料进行分析,采集患者标本进行细菌培养,采用改良K-B纸片琼脂扩散法进行药敏试验,试验结果根据NCCLS判定标准进行判读,分析感染性角膜炎患者病原菌分布及其耐药性。结果 87例感染性角膜炎患者中共分离出28株病原菌,其中革兰阳性球菌1 5株占53.58%,革兰阴性球菌1株占3.57%,革兰阳性杆菌3株占10.71%,革兰阴性杆菌9株占32.14%;革兰阳性球菌中表皮葡萄球菌对青霉素G、氨苄西林以及亚胺培南的耐药率,分别为83.33%、66.67%以及50.00%,金黄色葡萄球菌对青霉素G和亚胺培南的耐药率,分别为100.00%、66.67%,肺炎链球菌对青霉素G耐药率达100.00%;大肠埃希菌对氨苄西林和头孢呋辛的耐药率均为100.00%,铜绿假单胞菌的耐药率均>50.00%,对于氨苄西林耐药率达到100.00%,肺炎克雷伯菌对环丙沙星、头孢他啶以及头孢唑林耐药率均为0。结论感染性角膜炎患者病原菌类型较多,主要以葡萄球菌属和大肠埃希菌为主,治疗时应该加强病原菌监测,选择合理抗菌药物治疗,提高治愈率。OBJECTIVE To study pathogen distribution and drug resistance of infectious keratitis,so as to provide a basis for clinical treatment.METHODS Clinical data of 87 cases of infectious keratitis patients in the hospital from Dec.2012 to Dec.2015 were selected and analyzed.The specimens of patients were collected and cultured,drug sensitivity test was using a modified KB disk diffusion method according to NCCLS test results interpretation criteria,and the pathogen distribution and drug resistance of infectious keratitis were analyzed.RESULTS Totally 28 strains of pathogens were isolated from 87 cases of infectious keratitis patients,including 15 strains of gram-positive bacteria,accounting for 53.58%,and 1strain of gram-negative bacteria,accounting for 3.57%.3cases of gram-positive bacteria,10.71% 9gram-negative bacteria,accounting for 32.14%.The drug resistant rates of gram-positive bacteria Staphylococcus epidermidis to penicillin G,ampicillin and imipenem were 83.33%,66.67% and 50.00%,Staphylococcus aureus to penicillin G and imipenem were 100.00% and 66.67%,Streptococcus pneumoniae to penicillin G was 100.00%,E.coli to ampicillin and cefuroxime were both 100.00%,Pseudomonas aeruginosa to all drugs were over 50.00%,and to ampicillin reached 100.00%,Klebsiella pneumoniae to ciprofloxacin,cefazolin and ceftazidime were was all 0.CONCLUSIONPatients with infectious keratitis had many types of pathogens,mainly E.coli and S.aureus,and it should strengthen the monitoring of pathogens,and choose the right antibiotic therapy,to improve the cure rate.

关 键 词：感染性角膜炎 病原菌分布 耐药性 

分 类 号：R378[医药卫生—病原生物学]