MTX联合不同中药对Ⅱ型胶原诱导关节炎大鼠血清TNF-α及RORγt mRNA表达的影响  

Effects of integrated MTX and various Chinese materia medica on serum TNF-α and m RNA expression of RORγt in arthritis model rat induced by collagen

作　　者：田伟兰[1] 王威[1] 李睿[1] 翟华强[1,2] 何忠平[3] 梁晓琪 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京师范大学资源学院资源生态与中药资源研究所,北京100875 [3]北海国发海洋生物产业股份有限公司制药厂,广西北海536000

出　　处：《中华中医药杂志》2016年第10期4184-4187,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81373887);中国博士后科学基金面上项目(No.2012M510346)~~

摘　　要：目的:观察甲氨蝶呤(MTX)联合不同中药影响Ⅱ型胶原诱导关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)以及孤核受体m RNA(RORγt m RNA)表达的差异。方法:将Waster大鼠随机分为5组,建立Ⅱ型胶原诱导关节炎模型,正常组和模型组给予0.9%氯化钠溶液灌胃,各给药组分别给予对应药品,6周后,各组大鼠经腹主动脉取血,采用ELISA法检测大鼠中血清TNF-α,以荧光PCR法检测大鼠脾脏RORγt m RNA表达,评价MTX联合中药对类风湿性关节炎治疗差异。结果:与正常组比较,模型组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均有所升高(P<0.01);与模型组比较,各治疗组大鼠血清TNF-α及脾脏RORγt m RNA表达水平降低(P<0.01)。与MTX组比较,MTX+雷公藤组和MTX+海蛇药酒组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均明显降低(P<0.05)。结论:MTX、MTX联合雷公藤与海蛇药酒对CIA大鼠有一定的治疗作用,且MTX+雷公藤、MTX+海蛇药酒治疗效果最佳,能显著下调关节滑膜RORγt m RNA的表达,降低血清TNF-α水平,从而发挥抑制关节炎性反应的作用。Objective： Observed the impact of rheumatoid arthritis medicine MTX combination of different serum TNF-α expression in rats as well as differences in RORγt m RNA. Methods： The Waster rats were randomly divided into five groups, the normal group, model group, MTX group, MTX＋tripterygium group, MTX＋sea snake wine group, established type Ⅱ collagen-induced arthritis model in rats, the second booster immunization a week later, the normal group and model group received 0.9% sodium chlo-ride injection, corresponding to each treatment group were given the drug, administered continuously for two months, each group abdominal aorta blood, leaving the supernatant after centrifugation of blood, detected by ELISA rat serum TNF-α; rats in each group after taking blood, spleen, fluorescence PCR assay RORγt m RNA expression in rat spleen evaluate differences MTX combination of traditional Chinese medicine treatment of rheumatoid arthritis. Results： Compared with normal group, each group of rats serum TNF-α level and spleen RORγt m RNA expression was increased（P〈0.01）. Compared with model group, the treatment group rats serum TNF-α m RNA expression level and spleen RORγt lower（P〈0.01）. Compared with MTX group, MTX＋tripterygium wilfordii and MTX＋sea snake wine group rats serum level of TNF-α and spleen RORγt m RNA expression were significantly lower（P〈0.05）. Conclusion： MTX, MTX combination tripterygium and sea snake wine on collagen-induced arthritis in rats have a therapeutic effect, and MTX＋tripterygium, MTX＋sea snake wine optimal treatment, significantly reduced synovial RORγt m RNA expression, and decreased serum TNF-α levels, which play a role in inhibition of arthritis.

关 键 词：类风湿性关节炎 甲氨蝶呤 肿瘤坏死因子-α 孤核受体m RNA 

分 类 号：R684.3[医药卫生—骨科学]