右归饮通过Wnt/ERK信号通路交叉串扰影响成骨细胞相关蛋白的表达  

Yougui Drink's effect on the expression of osteoblast-related protein through crosstalk between Wnt/β-catenin and ERK signal pathway

作　　者：范连霞[1] 章建华[2] 尹华[1] 张成龙[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江中医药大学附属第一医院,杭州310053

出　　处：《中华中医药杂志》2016年第10期4249-4252,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81273772);浙江省中医药科技计划项目(No.2010ZA026);浙江省自然科学基金项目(No.Y2101299)~~

摘　　要：目的:观察细胞外信号调节激酶(ERK)、Wnt/β-连环蛋白(β-catenin)信号通路相关蛋白在右归饮含药血清影响成骨细胞过程中的作用,明确右归饮含药血清对ERK、Wnt通路和ERK/Wnt交叉串扰的作用。方法:体外培养成骨细胞,分组,加药干预。培养3d后,应用ELISA检测Wnt/β-catenin及ERK通路相关蛋白β-catenin、Runx2、ERK蛋白表达情况。结果:右归饮含药血清能显著上调3种蛋白表达(P<0.05,P<0.01)。联合阻断ERK、Wnt通路,3种蛋白表达水平较含药血清组有明显降低(P<0.05,P<0.01);与单独阻断其中一条通路比较,有明显下降(P<0.01)。单独阻断某一条通路,仅Runx2较右归饮含药血清组有明显降低(P<0.05,P<0.01)。结论:右归饮含药血清能显著增加成骨细胞活性,该作用与ERK、Wnt信号通路密切相关,且两条通路之间存在一定的交叉串扰作用。Objective： To investigate the influence of Wnt/β-catenin and ERK signal pathway in the effect of Youguiyin containing serum on osteoblast, and to elucidate the effects of Youguiyin containing serum on ERK, Wnt/β-catenin signal pathway and the crosstalk between the two pathways. Methods： The osteoblast（OB） from cranial bones of rat neonates were isolated and cultured for 3 days in vitro, which were divided into 5 groups. Levels of relevant proteins in Wnt/β-catenin and ERK signal pathway including β-catenin, ERK and Runx2 in each group were detected with ELISA assay. Results： Youguiyin containing serum could significantly upregulate the expression of β-catenin, ERK, Runx2 protein（P〈0.05, P〈0.01）. When ERK and Wnt/β-catenin signal pathway were blocked simultaneously, the level of β-catenin, ERK, Runx2 protein reduced significantly compared to the Youguiyin containing serum group（P〈0.05, P〈0.01）. The level of β-catenin, ERK, Runx2 protein reduced significantly compared to thesituation when either of the pathways was blocked. When block ERK signal pathway alone or Wnt/β-catenin signal pathway alone, only the level of Runx2 reduced significantly compared to the Youguiyin containing serum group（P〈0.05, P〈0.01）. Conclusion： Youguiyin containing serum could apparently increase activity of osteoblast. This action was closely correlated with ERK signal pathway and Wnt/β-catenin signal pathway. Meanwhile there must be some crosstalk effects between the two pathways.

关 键 词：WNT/Β-CATENIN信号通路 ERK信号通路 交叉串扰 右归饮 成骨细胞 

分 类 号：R285[医药卫生—中药学]