桉蝙蛾高毒力白僵菌菌株筛选及鉴定  

Selection and identification of a high virulent strain of Beauveria bassiana from Endoclyta signifer

作　　者：邹东霞[1] 史国英[2] 廖旺姣[1] 蒋学建[1] 吴耀军[1] 蓝霞[3] ZOU Dongxia et al(Key Laboratory of Central South Fast-growing Timber Cultivation of State Fores- try Administration, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning 530002, Chin)

机构地区：[1]广西壮族自治区林业科学研究院国家林业局中南速生材繁育实验室广西优良用材林资源培育重点实验室,广西南宁530002 [2]广西壮族自治区农业科学院微生物研究所,广西南宁530037 [3]广西大学农学院,广西南宁530005

出　　处：《中国森林病虫》2016年第5期1-4,共4页Forest Pest and Disease

基　　金：广西自然科学基金项目(2013GXNSFBA019100)"桉蝙蛾高毒力白僵菌筛选及菌制剂的研究";广西优良用材林资源培育重点实验室开放课题(12A0401)"桉蝙蛾高毒力白僵菌的鉴定及产孢条件的优化"

摘　　要：研究了分离自桉蝙蛾Endoclyta signifer Walke的6株白僵菌Beauveria spp.菌株的菌落生长、产孢量及孢子萌发率等生物学特性。结果表明,菌落生长最快的是菌株B-36,菌落直径为53.08±1.01 mm;产孢量最大的是B-77,产孢量为(29.80±1.95)×10~5个/mm^2;孢子萌发率最高的是B-41,GT_(50)值为13.74 h。对桉蝙蛾毒力最高的菌株是B-36,LT_(50)值为7.06 d。形态学及rDNA-ITS序列分析结果显示菌株B-36为球孢白僵菌Beauveria bassiana。The bionomics such as colony growth,spore yield and spore germination rate of 6 strains of Beauveria spp. isolated from Endoclyta signifer Walker were studied. The results showed the Colony of B-36 grewfastest with a diameter of 53. 08 ± 1. 01 mm,B-77 had the largest spore yield which was（ 29. 80 ± 1. 95） ×10^5 spores / mm^2,and the spore germination rate of B-41 was the highest with GT50 value of 13. 74 h. The laboratory toxicity test showed that B-36 was the toxicist strain with LT50 value of 7. 06 d. The morphology and r DNA-ITS sequence analysis showed that strain B-36 was Beauveria bassiana.

关 键 词：桉蝙蛾 球孢白僵菌 毒力 筛选 鉴定 

分 类 号：S763.42[农业科学—森林保护学]