土田七的组织培养与快速繁殖  

Tissue culture and rapid propagation of Stahlianthus involucratus

作　　者：黄赛[1] 符瑞侃 杨珺[1] 戚华沙[1] 吕德任 潘梅[1] 

机构地区：[1]海南省农业科学院热带园艺研究所,海口571100

出　　处：《植物生理学报》2016年第9期1347-1352,共6页Plant Physiology Journal

基　　金：海南省科研院所技术开发研究专项(KYYS-2015-14)~~

摘　　要：通过比较不同的外植体、基础培养基、植物生长调节剂种类和浓度配比、蔗糖浓度以及生根苗的移栽基质,建立土田七组织培养快速繁殖体系。结果表明,以茎尖为外植体诱导效果最好;培养基MS+6-BA 3.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+蔗糖40 g·L^(-1)利于形成丛生芽,30 d的增殖系数为4.16;MS+NAA 0.6~0.8 mg·L^(-1)适宜诱导生根获得再生植株,生根率100%;生根苗移栽于椰糠中成活率达100%。The techniques of tissue culture and rapid propagation of Stahlianthus involucratus were studied by comparing with different explants,basic medium,types and concentrations of plant growth regulators,sucrose concentration and transplanting medium.The results showed that the stem tips were the best explants.MS＋6-BA 3.0 mg·L-1＋NAA 0.2 mg·L-1＋sucrose 40 g·L-1 could effectively induce fasciculate buds,the propagation coefficient was 4.16 per 30 days;the most optimum rooting medium was MS＋NAA 0.6–0.8 mg·L-1,and the rooting rate was 100%.The rooting seedlings were effectively transplanted in coconut chaff,and the survival rate was 100%.

关 键 词：土田七 外植体 组织培养 快速繁殖 

分 类 号：S567.236[农业科学—中草药栽培]