西藏设施番茄晚疫病病原菌鉴定及生物学特性研究  

Research on the Identification and Biological Characteristics of Late Blight Pathogen of Greenhouse Tomato in Tibet

作　　者：相栋[1] 代万安[1] 巩文峰[2] 杨杰[1] 尼玛玉珍[1] 

机构地区：[1]西藏自治区农牧科学院蔬菜研究所,西藏拉萨850032 [2]西藏大学农牧学院,西藏林芝860000

出　　处：《北方园艺》2016年第19期114-118,共5页Northern Horticulture

基　　金：西藏自治区自然科学基金资助项目(13-43)

摘　　要：以具典型晚疫病症状的番茄病叶为试材,采用薯片夹叶分离法对病原菌进行分离,测定了不同培养基、pH、温度、碳源、氮源对菌丝生长、产孢的影响,通过病原菌鉴定和生物学特性研究,以期为西藏设施番茄晚疫病发生及防治提供理论依据。结果表明：引起设施番茄晚疫病的病原菌为致病疫霉菌Phytophthora infestans（Mont）de Bary。病菌菌丝在大多数培养基上均能良好生长,其中RSA培养基最适宜菌丝生长及产孢;菌丝生长和产孢的最适温度范围为18℃,最适生长pH 6-7;菌丝对碳源的利用,以可溶性淀粉最好,麦芽糖最差;在氮源利用方面,菌丝在以脯氨酸为氮源的查氏培养基上生长最快,在精氨酸、胱氨酸和氯化铵上不生长。孢子囊的致死温度为55℃,15min。With typical symptom of late blight of tomato disease leaves as test material, the pathogen was isolated by using the method of leaf claping with potato slices. The effects of different medium, pH, temperature, carbon source and nitrogen source on the mycelium growth and spore production were studied. Through pathogen identification and biological characteristics research, in order to provide a theoretical basis for the occurrence and control of late blight pathogen of greenhouse tomato in Tibet. The results showed the main pathogeny causing tomato late blight was identified as Phytophthora infestans（Mont） de Bary. Most of culture medium were conducive to the growth of mycelium,and RSA was the best one. The optimum temperature for mycelial growth and sporulation was 18℃, and the optimum pH was 6-7. The soluble starch was the best C source and maltose was the worst C source. Proline was the best N source while the mycelium was not grown on arginine, cystine and ammonium chloride. The sporangia lethal temperature was 55 ℃, 10 min.

关 键 词：番茄 晚疫病菌 分离 鉴定 生物学特性 

分 类 号：S436.412.12[农业科学—农业昆虫与害虫防治]