植物生长调节剂和蔗糖浓度对狼爪瓦松愈伤组织生物量及有效物质积累的影响  

Effect of Plant Growth Regulators and Sucrose Concentrations on the Proliferation,Growth and Effective Compounds Content of Orostachys cartilaginous Callus

作　　者：张万博 朴炫春[1] 杨帆[1] 姜银姬 廉美兰[1] 金英花[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《北方园艺》2016年第19期171-175,共5页Northern Horticulture

基　　金：吉林省博士后科研资助项目(RB201307)

摘　　要：以狼爪瓦松愈伤组织为试材,采用正交实验的方法,研究了MS基本培养基浓度及6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)2种植物生长调节剂对狼爪瓦松愈伤组织生物量和有效物质含量的影响;同时,对适宜的蔗糖浓度进行了筛选。结果表明:MS+3.5mg·L^(-1) 6-BA+0.1mg·L^(-1) NAA组合,最有利于愈伤组织生长和多糖、总酚及黄酮的积累;MS+3.5mg·L^(-1)6-BA+0.1mg·L^(-1) NAA培养基中加入30g·L^(-1)的蔗糖时,愈伤组织生长最佳,有效物质含量最高,在此蔗糖浓度下狼爪瓦松愈伤组织可生产711.2mg·L^(-1)多糖、165.1mg·L^(-1)总酚和154.5mg·L^(-1)黄酮。Orostachys cartilaginous callus was used as experiment material, the effect of MS strength and concentrations of plant growth regulators, 6-BA and NAA on callus biomass and effective compound accumulation was explored by orthogonal experiment method. The suitable sucrose concentration was also selected. The results showed that, MS medium supplemented with 3. 5 mg·L^-1 6-BA and 0. 1 mg·L^-1 NAA was favorable for callus growth and effective compound accumulation, the maxium fresh and dry weigth of calluses were found when the medium was supplied 30 g ·L^-1sucrose,711. 2 mg ·L^-1 of polysaccarides,165. 1 mg·L^-1 of phenolics and 154. 5 mg·L^-1of flavonoids were obtained in Orostachys cartilaginous callus.

关 键 词：狼爪瓦松愈伤组织 MS培养基 植物生长调节剂 蔗糖浓度 生物量 有效物质 

分 类 号：R282.71[医药卫生—中药学]