双三氟甲烷磺酰亚胺类离子液体对产紫青霉菌株全细胞催化特性的影响  

Effect of the Ionic Liquids with Anion Tf_2N^- on the Whole Cell Catalytic Properties of Penicillium Purpurogenum Li-3 Strain

作　　者：张馨[1] 李扬[1] 曹红[2] 欧阳巧凤 郑兰兰[1,3] 李春[4] Zhang Xin LI Yang CAO Hong Ouyang Qiaofeng Zheng Lanlan LI Chun(College of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China College of Chemistry and Environmental Science, Kashi University, Kashi 844006, China)

机构地区：[1]石河子大学化学化工学院,石河子832003 [2]嘉兴学院生物与化学工程学院,嘉兴314001 [3]喀什大学化学与环境科学学院,喀什844006 [4]北京理工大学生命学院,北京100081

出　　处：《高等学校化学学报》2016年第10期1870-1875,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21146012,21266029)资助~~

摘　　要：研究了双三氟甲烷磺酰亚胺阴离子Tf_2N^-分别与5种不同阳离子组成的离子液体对产紫青霉菌(Penicillium purpurogenum Li-3)的生长、代谢、细胞膜透性及全细胞催化活性的影响.结果表明,[N_(1,4,4,4)]Tf_2N对产紫青霉菌的生长有促进作用,[Py_(14)]Tf_2N,[Bmim]Tf_2N,[BPy]Tf_2N和[P_(6,4,4,4)]Tf_2N 4种离子液体对产紫青霉菌的生长则均有不同程度的抑制.代谢活力保留值R的测定结果表明,[P_(6,4,4,4)]Tf_2N和[N_(1,4,4,4)]Tf_2N对产紫青霉菌体细胞表现出相对较高的生物相容性;5种离子液体对菌体细胞的细胞膜透性均有改善作用.全细胞催化反应数据显示,最优离子液体为[P_(y14)]Tf_2N,当其加入量为25%,反应84 h后,单葡萄糖醛酸基甘草次酸(GAMG)产率高达95.38%.5种离子液体对产紫青霉菌的生长、代谢、细胞膜透性及全细胞催化活性的影响不仅与阴离子Tf_2N^-有关,阳离子的组成、结构和性质也发挥重要的作用.Penicillium purpurogenum Li-3 cells properties of growth, metabolism, permeability of cell membrane and catalysis were studied in five ionic liquids composed with anion Tf2N^- and five different cations. The results showed that,the ionic liquids [Py14]Tf2N,[Bmim]Tf2N,[BPy]Tf2N and [P6,4,4,4]Tf2N had inhibitory effect on the growth of penicillium purpurogenum Li-3 while ionic liquid [N1,4,4,4]Tf2N had a promoting effect. Through metabolic activity determination of retention value（ R） in ionic liquids,[P6,4,4,4]Tf2N and [N1,4,4,4]Tf2N showed relatively high biocompatibility to cells. At the same time,five ionic liquids on cell membrane permeability were improved effect. Whole-cell catalysis results displayed that[Py14]Tf2N was optimal ionic liquid. When the addition of [Py14]Tf2N was 25%,after 84 h,glycyrrhetinic acid monoglucuronide（ GAMG） rate and glycyrrhizin acid（ GL） conversion rate were as high as 95. 38% and99. 84%. Therefore,the addition of [Py14]Tf2N,greatly promoted the formation of conversion and product. It showed that the effects of five kinds of ionic liquids on cells properties of growth and metabolism,cell membrane permeability and the whole cell catalytic activity were not only related to the anion Tf2N^-,but also played an important role in the composition,structure and properties of the cations.

关 键 词：双三氟甲烷磺酰亚胺阴离子 阳离子 离子液体 产紫青霉菌 单葡萄糖醛酸基甘草次酸 

分 类 号：O643[理学—物理化学] Q556.2[理学—化学]