吉林地区猪链球菌的分离及快速检测方法的建立  

Isolation and Rapid Detection of Streptococcus suis in Jilin District

作　　者：宋永利[1] 尹柏双[1] 付连军[1] 刘立明[1] 沙万理 王奔[1] 李静姬[1] 

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132101

出　　处：《安徽农业科学》2016年第25期131-132,共2页Journal of Anhui Agricultural Sciences

基　　金：吉林农业科技学院重点学科培育项目[吉农院合字(2013)第X028号]

摘　　要：从吉林市周边地区8个猪场收集病料,采集疑似猪链球菌的猪样品,进行病原菌的分离、生化试验、动物致病性试验和毒力因子的PCR检测。共分离到8株分离株,其中3株有致病性,5株无致病性。PCR能够高效、快速诊断出该病菌是否有致病性。该试验结果可为正确及时诊断治疗链球菌病提供理论依据。Suspected samples of Streptococcus sui were collected from 8 pig farms in the surrounding areas of Jilin City.The isolation, bio-chemical test, animal pathogenicity test and the virulence factor PCR test of pathogenic bacteria were carried out.A total of eight strains were isolated.And among them, 3 strains had pathogenicity, others were non-pathogenic.RCR could rapidly and efficiently diagnose whether the strain had pathogenicity.This research provided theoretical basis for the correct and timely treatment of Streptococcus suis.

关 键 词：猪链球菌 分离 生化试验 动物致病性试验 PCR检测 

分 类 号：S851.347[农业科学—预防兽医学]