PVDF微孔滤膜负载金纳米粒子用于牛奶中三聚氰胺的SERS快速检测  

Rapid Detection of Melamine in Milk by Surface-Enhanced Raman Spectroscopy with PVDF Membranes as Supports

作　　者：曾甜[1] 陈钱[1] 江茜[1] 周吉[1] 叶勇[1] ZENG Tian CHEN Qian JIANG Qian ZHOU Ji YE Yong(Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan 430062, P. R. Chin)

机构地区：[1]有机化工新材料湖北省协同创新中心,湖北大学有机功能分子合成与应用教育部重点实验室,武汉430062

出　　处：《光散射学报》2016年第3期209-213,共5页The Journal of Light Scattering

基　　金：国家自然科学基金(21003034);湖北省教育厅团队项目(T201101);湖北省教育厅青年人才项目(Q20131002)

摘　　要：本文报道了一种利用聚偏二氟乙烯(Polyviny Lidene Fluoride,PVDF)微孔滤膜为载体的三聚氰胺(Melamine,MAM)的简便快速痕量分析技术。通过柠檬酸钠还原法制得平均粒径为30nm的纳米金溶胶,加入不同浓度的三聚氰胺后金纳米粒子快速聚集,这种纳米金聚集体可以通过简便快捷的过滤技术截留在PVDF微孔滤膜表面,进而用于SERS检测,在水溶液中最低检测浓度为0.05mg/L。我们进一步在牛奶样品中进行检测,在牛奶中检测限可以达到1mg/L,满足大部分国家规定的婴儿配方食品中三聚氰胺的限量值为1mg/kg的参考标准。该分析方法制备过程简单、成本低廉、总分析时间短,易于实现现场快速灵敏检测,应用前景广阔。We demonstrate an extremely simple and practical surface-enhanced Raman spectroscopy（SERS）technique for trace melamine detection in milk with PVDF（Polyviny Lidene Fluoride）membranes as supports.Through trisodium citrate reduction method,gold nanoparticles with average diameter of 30 nm were obtained.Upon addition to trace-level melamine,gold nanoparticle solution exhibits a highly sensitive colour change from red to blue and rapid aggregation behavior within several minutes.The SERS detection platform was constructed after trapping the mixture of aggregates and melamine on PVDF membrane through a simple filter-based approach,and the lowest detectable concentration was 0.05mg/L in aqueous solution.Moreover,for the melamine-spiked milk samples,the lowest detectable concentration was 1mg/L,which could meet the requirement accepted by most countries that the threshold in infant formula was 1ppm.Thus,due to the simple procedure,the low-cost of the substrates and the short total analysis time,our technique enables SERS to be practical for a broad range of analytical applications,including field-based detection of toxins in large volume samples.

关 键 词：表面增强拉曼散射 PVDF微孔滤膜 金纳米粒子 三聚氰胺 

分 类 号：O657[理学—分析化学]