退黄藤中东莨菪内酯的分离纯化及含量测定  

Isolation,purification and determination of scopoletin in Pegia sarmentosa

作　　者：黄建猷[1] 卢文杰[1] 谭晓[1] 陆国寿[1] 黄周锋[1] 兰保强[1] 

机构地区：[1]广西中医药研究院广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《华西药学杂志》2016年第5期467-469,共3页West China Journal of Pharmaceutical Sciences

基　　金：广西中药质量标准研究重点实验室系统性研究课题(桂中重系201307)

摘　　要：目的分离纯化退黄藤中的东莨菪内酯并测定其含量。方法采用硅胶柱色谱分离纯化退黄藤中的东莨菪内酯,并根据理化性质及光谱数据鉴定其结构;采用HPLC法测定其含量,色谱柱为Zorbax Eclipse XDB-C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%冰乙酸(39∶61),流速1.0 m L·min^(-1),检测波长345 nm。结果东莨菪内酯3.11~31.1μg·m L^(-1)与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.29%,RSD=1.40%(n=6)。结论所用方法简便、准确、快速,可用于退黄藤药材的质量控制。OBJECTIVE To isolate,purify and determine the scopoletin in Pegia sarmentosa （ Lecte. ） Hand. - Mazz.. METHODS Scopoletin was isolated from P. sarmentosa by the silica gel column chromatography,and its structure was identified on the basis of spectral characteristics. Zorbax Eclipse XDB - C18 （250 mm×4.6 mm,5 μm） reversed - phase column was used with mobile phase as methyl alcohol -0. 1% acetic acid solution（39 ： 61 ）. The flow rate was 1.0 mL·min^-1 with the detection wavelength of 345 nm. RESULTS The linear range for scopoletin was 3.11 -31.1 μg·mL^-1 （r =0. 9999）. The average recovery was 99.29% with the RSD of 1.40% （n = 6）. CONCLUSION The method is simple,accurate and rapid,and it can be used for quality control of P. sarmentosa.

关 键 词：退黄藤 东莨菪内酯 香豆素 化学成分 分离纯化 高效液相色谱法 质量控制 

分 类 号：R28[医药卫生—中药学]