HPLC测定牡丹皮中的三没食子酰葡萄糖和五没食子酰葡萄糖  

Determination of trigalloyl glucose and pentagalloyl glucose in Moutan cortex by HPLC

作　　者：章丽[1,2,3] 顾俊菲[1] 袁嘉瑞 赵冰洁[1] 汪春飞 封亮[1,3] 贾晓斌[1,3] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013 [3]中国中医科学院道地药材国家重点实验室培育基地,北京100700

出　　处：《华西药学杂志》2016年第5期509-511,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81473394);江苏省自然科学基金资助项目(BK2012491);中央本级重大增减支项目(2060302)

摘　　要：目的采用HPLC法测定牡丹皮中三没食子酰葡萄糖和五没食子酰葡萄糖的含量。方法采用Agilent TC-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸,梯度洗脱,流速0.8 m L·min^(-1),检测波长274 nm,柱温25℃,进样量10μL。结果三没食子酰葡萄糖0.068~0.680μg(r=0.9994)、五没食子酰葡萄糖0.604~6.040μg(r=0.9997)与峰面积呈良好的线性关系,二者的平均加样回收率分别为99.31%、99.11%,RSD分别为1.48%、1.22%(n=9)。结论所用方法简便、准确、稳定、灵敏度高,可用于牡丹皮药材的质量控制。OBJECTIVE To detect trigalloyi glucose and pentagalloyl glucose in Moutan cortex by HPLC. METHODS Agilent TC - C18 column（250 mm×4.6 mm,5 μm） was adopted. The mobile phase consisted of acetonitrile and 0.1% aqueous formic acid with gradient elution at a flow - rate of 0.8 mL·min^-1. The detection wavelength was 274 nm with the injection volume of 10 μL and the column temperature of 25 ℃. RESULTS That trigalloyi glucose and pentagalloyl had a good linearity between 0.068 - 0.680 μg （ r = 0.9994） and 0. 604 - 6. 040 μg （ r = 0. 9997 ）, respectively. The average recovery rates of them were 99.31% with the RSD of 1.48% （ n = 9 ） and 99.11% with the RSD of 1.22% （ n = 9 ）, respectively. CONCLUSION The method is simple, accurate and highly sensitive, and it is suitable for the quality control of Moutan cortex.

关 键 词：牡丹皮 三没食子酰葡萄糖 五没食子酰葡萄糖 高效液相色谱法 含量测定 质量控制 鞣质 

分 类 号：R917[医药卫生—药物分析学]