GC-MS法分析青海省不同产地冬虫夏草中的脂肪酸  

Analysis of fatty acids in Cordyceps sinensis from the different regions of Qinghai province by GC-MS

作　　者：肖远灿[1] 胡风祖[1] 迟晓峰[1] 董琦[1] 张波[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008

出　　处：《华西药学杂志》2016年第5期517-520,共4页West China Journal of Pharmaceutical Sciences

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26);青海省科技计划应用基础项目(2011-Z-716)

摘　　要：目的分析比较青海省4个地区7个不同产地的11批冬虫夏草中的脂肪酸类成分。方法采用一步皂化甲酯化法衍生冬虫夏草中的脂肪酸类成分,GC-MS法和对照品对照定性、定量分析脂肪酸的组成和含量。结果青海省产的冬虫夏草中主要含有C_(14)~C_(20)的13种脂肪酸,其总量为67.376~148.494 mg·g^(-1);其中,棕榈酸、油酸、亚油酸等3种脂肪酸的含量较高,其总含量占13种脂肪酸总量的92.74%~96.23%;同一样品中,不饱和脂肪酸与饱和脂肪酸的比例为3.08~7.71;C_(18)和C_(16)的脂肪酸种类最多、含量最高,C_(18)/C_(16)为3.05~8.18;C_(18)脂肪酸中C_(18∶1)/C_(18∶2)为1.10~2.91。结论青海省不同产地的冬虫夏草样品中13种脂肪酸的组成差异较小,但脂肪酸的含量和比例差异较大。OBJECTIVE To analyze and compare the fatty acid constitutes of 11 batches of Cordyceps sinensis from 7 different origins in 4 regions of Qinghai province. METHODS Fatty acid was derivated by one - step esterificating. Then a GC - MS method with reference substances was used to ascertain the composition and content of C. sinends samples. RESULTS 13 kinds of fatty acid of 14 carbon - 20 carbon were found in C. sinensis from Qinghai province, whose total amount was between 67. 376 - 148. 494 mg·g^-1. Futhermore,palmitic acid, oleic acid and linoleic acid were the highest in the content. And the total content of the above three kinds of fatty acid accounted for 92.74% - 96.23% in all 13 fatty acids. In the same sample, the ratio of UFA/SFA was 3.08 - 7.71. The fatty acids of 18 carbon and 16 carbon were the higher components than the other fatty acids, while the 18 carbon fatty acids were the highest;and the ratio（ C18/C16 ） ranged from 3.05 to 8.18. Additionally, the ratio of C18： 1/C18： 2 was in the range of 1.10 to 2.91. CONCLUSION The differences of the composition of the above 13 kinds of fatty acid in C. sinensis from the different regions of Qinghai province were small,but large difference was exsited in the content and proportion of fatty acids.

关 键 词：冬虫夏草 青海 不同产地 脂肪酸 气相色谱-质谱联用法 定性 定量 对比分析 

分 类 号：R917[医药卫生—药物分析学]