家兔XKR4基因遗传多态性及其与生长性能的相关性  

Polymorphisms of rabbit XKR4 gene and its association with growth traits

作　　者：吴周林 曾予[1] 贾先波[1] 陈仕毅[1] 王杰[1] 赖松家[1] WU Zhoulin ZENG Yu JIA Xianbo CHEN Shiyi WANG Jie LAI Songjia(College of Animal Science and Technology ,Sichuan Agricultural University ,Wenjiang ,Sichuan 611130 ,China Department of Environment and Life Science ,Sichuan Minzu College ,Kangding, Sichuan 626001 ,China)

机构地区：[1]四川农业大学动物科技学院,四川温江611130 [2]四川民族学院环境与生命科学系,四川康定626001

出　　处：《西北农林科技大学学报（自然科学版）》2016年第8期13-18,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：四川省"十二五"畜禽育种攻关项目(2011NZ0099-4);国家现代农业技术体系项目(CARS-44-A-2)

摘　　要：【目的】研究家兔XKR4基因的遗传多态性,并分析其与家兔生长速度的相关性。【方法】以新西兰兔(184只)和伊拉兔(193只)为研究材料,取其耳部组织,提取DNA,PCR扩增XKR4基因,采用直接测序法寻找CDS区单核苷酸多态性(SNP)位点,利用HRM技术对突变位点进行分型,并分析其与生长性状的相关性。【结果】在家兔XKR4基因内检测到3个SNP位点(内含子1中的c.804-8C>T及外显子3中的c.1668C>T和c.1698C>T)。针对外显子3中的2个SNP位点,对40个样本直接测序,结果发现仅存在CC和CT 2种基因型,且处于完全连锁状态。对2种兔突变位点的群体遗传参数分析发现其总杂合度为0.287 1,总有效等位基因数为1.402 7,多态性属于中等多态,表明该群体在该位点的遗传变异较高,有较大的选择潜力,可以利用该位点进行与生长性状相关的标记辅助选择。关联性分析发现,CTCT单倍型个体70日龄体质量显著高于CCCC单倍型个体(P<0.05),且35到70日龄平均日增重极显著高于CCCC单倍型个体(P<0.01)。【结论】XKR4基因可作为影响家兔生长性状的候选基因。【Objective】This work aimed to investigate the polymorphisms of rabbit XKR4 gene and its association with growth traits.【Method】New Zealand（184）and Ira（193）rabbits were selected.Genomic DNA was extracted from ear tissues for studying polymorphism of XKR4 gene by PCR.The polymorphisms in CDS of XKR4 were identified by direct sequencing,and the genotypes of candidate single nucleotide polymorphisms（SNPs）were confirmed by HRM method.Furthermore,the correlations with growth traits were analyzed.【Result】A total of three SNPs（c.804-8C〉T,c.1668C〉T and c.1698C〉T）were identified.For the two coding SNPs,there were two genotypes of CC and CT,which were completely linked by direct sequencing of 40 random rabbits from the two breeds.The total heterozygosity was 0.287 1and the effective number of alleles was 1.402 7.The polymorphism information was at middle level in the two rabbit populations,indicating that the rabbit population had sufficient diversity for selection and improvement of production traits,and can be treated as markers.The association analysis revealed the individuals ofCTCT haplotype had significantly higher body weight at 70 days than that of CCCC（P〈0.05）,and the average daily weight gain from 35 to 70days was extremely significantly higher（P〈0.01）.【Conclusion】XKR4is one of the candidate genes affecting growth traits of rabbits.

关 键 词：XKR4基因 单核苷酸多态性 生长速度 家兔 关联性分析 

分 类 号：S829.1[农业科学—畜牧学]