电针下合穴对急性胃黏膜损伤模型大鼠HMGB1及nAchR α7的对比研究  

Comparison of electroacupuncture at lower he-sea points on HMGB 1 and nAchR α7 in rats with acute gastric mucosal lesion

作　　者：张泓[1] 吴金峰[1] 祁芳[1] 尹秀婷 艾坤[1] 易细芹 凌希[1] 邓石峰 

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208

出　　处：《中国针灸》2016年第10期1071-1076,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81173327

摘　　要：目的:观察分别电针急性胃黏膜损伤(AGML)模型大鼠胃、大肠、小肠、胆腑下合穴后对白介素-1β(IL-1β)、高迁移率族蛋白1(HMGB1)及烟碱型乙酰胆碱受体α7(nAchRα7)的影响,探讨干预足三里治疗胃腑病变是否存在相对特异性。方法:将60只健康SD大鼠随机分为空白组、模型组、足三里组、上巨虚组、下巨虚组及阳陵泉组共6组,每组10只,雌雄各半。除空白组外均采用WRS法诱导建立急性胃黏膜损伤大鼠模型,空白组常规饲养,模型组束缚于鼠板上30min/次,足三里、上巨虚、下巨虚及阳陵泉组分别针刺与组名相应的双侧穴位并接电子针疗仪(疏密波10Hz/50Hz,左侧接正极,右侧接负极,强度以大鼠后肢末端轻微抖动为宜)治疗30 min,每天1次,连续治疗10d后,取胃组织,肉眼观察胃黏膜损伤情况并评分,ELISA法检测血清IL-1β及组织HMGB1含量,Western blot法检测nAchRα7的表达。结果:(1)与模型组比较,余组胃黏膜损伤指数(UI)、血清IL-1β及胃组织HMGB1含量均较低,且胃组织nAchRα7表达均较高(P<0.05,P<0.01);(2)与足三里组比较,上巨虚、下巨虚、阳陵泉组胃组织UI及血清IL-1β和胃HMGB1水平均较高(P<0.05,P<0.01),且阳陵泉组nAchRα7表达明显偏低(P<0.01)。结论:(1)电针与消化系统相关的内腑下合穴均可通过IL-1β、HMGB1及nAchRα7等调节机体免疫应答、减轻炎性反应、降低黏膜损伤,实现对AGML模型大鼠的干预治疗作用;(2)对比可知:足三里组对AGML模型大鼠的总体干预效应优于其他组,部分说明足三里穴与对应胃腑之间存在相对特异性。Objective To observe the influence of electroacupuncture（EA）at lower he-sea points of stomach,large intestine,small intestine and gallbladder on interleukin-1β（IL-1β）,high mobility group protein 1（HMGB 1）and alpha 7nicotinic acetylcholine receptor（nAchRα7）in rats with acute gastric mucosal lesion（AGML）,so as to explore whether there is relative specificity in treating gastric viscera disease by stimulating Zusanli（ST 36）.Methods Sixty healthy SD rats were randomly assigned into a blank group,a model group,a Zusanli group,a Shangjuxu group,a Xiajuxu group and a Yanglingquan group,ten rats in each one（half male and half female）.The WRS method was applied to induce the AGML model except the rats in the blank group.The rats in the blank group were treated with routine diet;the rats in the model group were treated with immobilization at rat platform,30 min per time;the rats in the Zusanli group,Shangjuxu group,Xiajuxu group and Yanglingquan group were treated with acupuncture and connected with EA device（dilatational wave 10Hz/50 Hz,positive electrode on the left side and negative electrode on the right side,intensity was appropriate when rat hind leg slightly shook）,30 min per time.The treatment was given once a day.After consecutive 10-day treatment,the gastric tissue was collected and the damage of gastric mucosa was evaluated;ELISA method was applied to measure the content of serum IL-1βand tissue HMGB 1;the Western blot method was applied to measure the expression of nAchRα7receptor.Results（1）Compared with the model group,the ulcer index（UI）of gastric mucosa,serum IL-1βand tissue HMGB 1were lower,and the expression of nAchRα7was increased in the remaining groups（P0.05,P0.01）.（2）Compared with the Zusanli group,the UI of gastric tissue,serum IL-1βand tissue HMGB 1were higher in the Shangjuxu group,Xiajuxu group and Yanglingquan group（P0.05,P0.01）,and the expression of nAchRα7was reduced in the Yanglingquan group（P0.01）.Conclusion（1）EA at digestive

关 键 词：电针 下合穴 AGML模型大鼠 IL-1β HMGB1 nAchRα7 合治内腑 

分 类 号：R246.1[医药卫生—针灸推拿学]