地塞米松联合葛根素治疗急性百草枯中毒大鼠的实验研究  

An experimental study on dexamethasone combined with puerarin in treatment of acute paraquat-poisoned rats

作　　者：陈贵洪 刘世平[2] 曹小平[2] 钟武[1] 陈睦虎[1] 张文[3] 

机构地区：[1]西南医科大学附属医院急诊医学部,四川泸州646000 [2]川北医学院附属医院,四川南充637000 [3]泸州市中医医院,四川泸州646000

出　　处：《中国中西医结合急救杂志》2016年第5期499-503,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：四川省卫生厅科研课题（川卫办发[2010]493）

摘　　要：目的：探讨地塞米松联合葛根素治疗百草枯（PQ）中毒的疗效并阐述其机制。方法30只SD大鼠按随机数字表法分为对照组、模型组、地塞米松组、葛根素组和联合治疗组，每组6只。采用腹腔注射PQ 25 mg/kg （1 mL）制备大鼠中毒模型；对照组腹腔注射1 mL生理盐水。2 h后地塞米松组腹腔注射20 mg/kg地塞米松1.5 mL；葛根素组腹腔注射100 mg/kg葛根素1.5 mL；联合治疗组则予以20 mg/kg地塞米松和100 mg/kg葛根素总量1.5 mL腹腔注射；对照组和模型组腹腔注射生理盐水1.5 mL。26 h时，重复上述治疗1次。收集各组大鼠制模后26～50 h尿液，检测尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）；制模后50 h采集腹主动脉血检测氧分压（PaO2）；取肺、肾组织测定肺系数、肾系数和左肺、肾组织匀浆丙二醛（MDA）、超氧化物岐化酶（SOD）、髓过氧化物酶（MPO）水平；取右肺、肾组织光镜下观察病理学改变。另取30只SD大鼠给予相同干预措施，观察各组大鼠30 d内的存活情况。结果模型组肺系数、肾系数、尿NAG及肺、肾组织匀浆中MDA、MPO均显著高于对照组〔肺系数：9.80±1.83比4.97±1.14，肾系数：9.40±1.32比7.01±0.32， NAG（U·kg-1·d-1）：1.93±0.18比0.41±0.03，肺MDA（nmol/mg）：1.04±0.15比0.28±0.10，肾MDA（nmol/mg）：1.39±0.16比0.66±0.13，肺MPO（U/g）：1.14±0.08比0.81±0.06，肾（U/g）：0.88±0.08比0.52±0.12〕；PaO2〔mmHg（1 mmHg＝0.133 kPa）：59.83±4.40比97.00±2.83〕和SOD（U/mg：肺为27.38±3.48比86.88±5.88，肾为24.18±3.74比57.86±6.14）显著低于对照组（均P＜0.05）。给予药物治疗后，葛根素组肺系数（7.21±1.87）、尿NAG（1.01±0.21）和肺、肾组织MDA（肺：0.49±0.09，肾：0.85±0.08）、MPO（肺：0.97±0.07，肾：0.68±0.10）均较模型组显著降低，PaO2（82.17±5.38）、SOD（肺：68.99±6.51，肾：37.90±3.62）均较模型组显著升高（P＜0.05�ObjectiveTo explore the therapeutic effect and mechanism of dexamethasone combined with puerarin for treatment of paraquat （PQ） poisoned rats.Methods Thirty Sprague Dawley （SD） rats were divided by random number table into 5 groups： control, model, dexamethasone, puerarin and combined groups, 6 rats in each group. The PQ poisoned rat model was established by intraperitoneal injection of PQ 25 mg/kg （1 mL）, while in the rat of the control group, the same volume of normal saline was injected intraperitoneally. After 2 hours, in the rat of dexamethasone group, 20 mg/kg dexamethasone in 1.5 mL was injected intraperitoneally, and in the rat of puerarin group, 100 mg/kg puerarin in 1.5 mL was injected intraperitoneally. In the rat of combined group, 20 mg/kg dexamethasone combined with 100 mg/kg puerarin in a total amount of 1.5 mL was injected intraperitoneally. In the control group and model group, 1.5 mL of normal saline was injected intraperitoneally. The above mentioned treatments were repeated once more 26 hours later. In 26-50 hours after modeling, urine was collected, and urine N-acetyl-beta-D-amino glycosidase enzymes （NAG） was tested; at the 50th, abdominal aortic blood was collected to test oxygen partial pressure （PaO2）. The lung index, kidney index, and the levels of malonaldehyde （MDA）, superoxide dismutase （SOD） and myeloperoxidase （MPO） were measured in the left lung and kidney tissue homogenates separately; the right lung and kidney were harvested to observe pathological changes under light microscope. Another 30 SD rats were treated the same as above measures but no sacrifice to observe 30-day survival rate in each group.Results The lung index, kidney index, NAG in urine, MDA and MPO levels in lung and kidney tissue homogenates in model group were significantly higher than those in the control group [lung index： 9.80±1.83 vs. 4.97±1.14, kidney index： 9.40±1.32 vs. 7.01±0.32, NAG （U·kg-1·day-1）： 1.93±0.18 vs. 0.41±0.03, MDA of lung （nmol/mg）： 1

关 键 词：百草枯 中毒 地塞米松 葛根素 丙二醛 

分 类 号：R595.4[医药卫生—内科学]