一株野大麦内生真菌的生物学与生理学特性  

Biological and physiological characteristics of Epichloё bromicola endophyte symbiotic with Hordeum brevisubulatum

作　　者：陈泰祥[1] 李春杰[1] 李秀璋[1] 

机构地区：[1]草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2016年第9期1658-1664,共7页Pratacultural Science

基　　金：国家重点基础研究发展规划(“973”计划)(2014CB138702);国家自然科学基金(31372366);教育部创新团队发展计划(IRT13019);兰州大学中央高校基本业务费专项资金(lzujbky-2016-180)

摘　　要：本研究对野大麦（Hordeum brevisubulatum）内生真菌（Epichlobromicola）菌株WBE1的生物学与生理学特性进行了研究。结果表明,内生真菌WBE1最适生长温度为22-25℃,4℃和35℃停止生长,最适生长pH为5.09-6.10的中性偏酸环境,对碳源的利用能力由强到弱依次为麦芽糖〉D-山梨糖〉甘露醇〉蔗糖〉乳糖〉可溶性淀粉〉葡萄糖〉D-果糖,D-木糖抑制其生长;对可利用氮源的利用能力由强到弱依次为胰蛋白胨〉牛肉膏〉蛋白胨〉酵母浸粉〉L-甲硫氨酸〉L-苯丙氨酸〉亮氨酸,不能利用硝酸钾、脯氨酸、草酸铵,钼酸铵抑制其生长,光照对菌丝生长无影响,CMA、OMA、PSA和PDA是菌丝生长较适宜的培养基,在WBA培养基上40d左右开始生长。菌株在PDA的前6周的生长速率呈增长趋势,第6周达最大值,第7周开始生长速率逐渐变小。Biological and physiological characteristics of endophyte Epichloё bromicola strain WBE1 isolated from wild barley seeds were measured in a range of temperatures,pH,light,carbon source,nitrogen source and growth rate values.WBE1 was able to grow at temperate from 10℃to 30℃ with the optimal temperature of 22-25 ℃ and stop growing at 4℃and 35℃.The optimal pH for mycelial growth ranged 5.09 to 6.1.The ability of carbon source utilization decreased in the following order：maltose 〉D-sorbose〉 mannose sucrose 〉lactose 〉starch 〉glucose.However,D-xylose inhibited the mycelial growth.The ability of nitrogen source utilization decreased in the following order：casein tryptone〉 beef extract 〉peptone〉 yeast 〉L-methionine〉 L-phenylalanine〉 leucine.However,potassium nitrate,praline and ammonium oxalate cannot be utilized by WBE1 and ammonium molybdate inhibited mycelial growth.Potato dextrose agar（PDA）,oat meal agar（OMA）,corn meal agar（CMA）and potato sugar agar（PSA）were optimal medium for WBE1.The mycelial growth rate on PDA reached maximum at the sixth week,then decreased gradually afterwards.

关 键 词：野大麦 内生真菌 生物学特性 生理学特性 

分 类 号：S543.9[农业科学—作物学] Q93[生物学—微生物学]