雷公藤多苷对胶原诱导关节炎大鼠滑膜肥大细胞浸润和活化的影响  

Effects of Tripterygium Wilfordii Polyglucosideon Infiltration and Activation of Mast Cells in Synoviumof Rats with Collagen-Induced Arthritis

作　　者：柳丽[1] 顾亚琴[1] 关乃富[2] 王晶晶[1] 付敏丽 周晓鹰[1] 

机构地区：[1]常州大学制药与生命科学学院,江苏常州213164 [2]常州市第二人民医院,江苏常州213003

出　　处：《常州大学学报（自然科学版）》2016年第5期82-86,共5页Journal of Changzhou University：Natural Science Edition

基　　金：江苏省高校自然科学研究面上项目(15KJB360001)

摘　　要：观察雷公藤多苷(GTW)对胶原诱导关节炎(CIA)大鼠滑膜肥大细胞数量和类胰蛋白酶水平的影响。采用牛Ⅱ型胶原蛋白诱导建立CIA大鼠模型后,分设正常组、模型组和GTW组(50mg/(kg/d),ig),正常组和模型组给予等体积溶媒,每天1次,连续28d。监测大鼠的关节炎指数评分,HE染色评价滑膜组织病理学改变,俾士麦棕染色观察滑膜肥大细胞浸润情况,ELISA检测滑膜组织类胰蛋白酶水平。结果显示,GTW可以显著降低CIA大鼠关节肿胀度,减轻滑膜炎症和关节破坏,减少CIA大鼠滑膜肥大细胞浸润,降低滑膜组织类胰蛋白酶水平。因此,GTW可以通过减少滑膜肥大细胞浸润、抑制其活化发挥抗RA作用。The study was aimed to observe the effects of Tripterygium wilfordii polyglycoside （GTW） on the number of mast cells and the level of tryptase in synovium of rats with collagen-induced arthritis （CIA）. Rat arthritis was induced by bovine type-II collagen. The rats were randomly divided into normal control group, model group and GTW group, which received intragastric administration daily of dissolvant or GTW （50mg/kg） for 28 days. The arthritis index was assessed and histological examinations were per- formed by hematoxylin andeosin staining. Infiltrationof synovial mast cells was determined by bismarck brown staining, and tryptase levels in synovial tissue were measured by enzyme-linked immunesorbent as- say. Compared with those in model group, the macroscopic joints welling, microscopic joint erosions, syn- ovial inflammation and mast cells infiltration were reduced significantly after GTW treatment. In addition, ELISA results showed that tryptase was decreased obviously as well. The results demonstrated that GTW effectively ameliorated synovial inflammation by inhibiting infiltration and activation of mast cells in syno- vium.

关 键 词：雷公藤多苷 胶原诱导关节炎 肥大细胞 类胰蛋白酶 

分 类 号：R967[医药卫生—药理学]