糖尿病大鼠阴茎海绵体硫化氢含量及其合成酶表达的变化  

Changes of hydrogen sulfide concentrations and synthetase expressions in the corpus cavernosum of diabetic rats

作　　者：赵蕙琛 柴家超 张亮[5] 袁明振[6] 彭力[7] 刘元涛 

机构地区：[1]青岛市立医院内分泌科,山东青岛266071 [2]山东大学第二医院肾内科,山东济南250033 [3]山东大学附属省立医院小儿外科,山东济南250021 [4]青岛市妇女儿童医院普外科,山东青岛266011 [5]山东大学第二医院内分泌科,山东济南250033 [6]山东大学第二医院泌尿外科,山东济南250033 [7]济南市第四人民医院干部保健病房,山东济南250031

出　　处：《山东大学学报（医学版）》2016年第10期25-28,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展计划(2010GWZ20248)

摘　　要：目的观察糖尿病大鼠及非糖尿病大鼠阴茎海绵体组织中硫化氢(H2S)含量及胱硫醚-γ-裂解酶(CSE)表达的差异。方法将Wistar大鼠随机平均分为两组,一组高脂饲料喂养8周后,注射链脲佐菌素(STZ)制备2型糖尿病大鼠模型(糖尿病组),另一组普通饲料喂养(对照组)。继续喂养12周后分离两组大鼠海绵体组织,检测海绵体组织内源性H2S的生成;采用RT-PCR法检测两组海绵体组织中CSE mRNA的表达;采用Western blotting法检测两组CSE蛋白的表达。结果糖尿病组内源性H2S浓度较对照组显著降低(P<0.01);糖尿病组海绵体组织中CSE mRNA和CSE蛋白的表达量均较对照组显著降低(P<0.01)。结论糖尿病大鼠阴茎海绵体H2S含量显著降低,其机制可能与CSE mRNA及蛋白水平表达减少有关。Objective To examine the endogenous hydrogen sulfide（ H2S） concentrations and expressions of cystathionine-γ-lyase（ CSE） in the corpus cavernosum tissues of diabetic and non-diabetic rats. Methods Rats were randomly divided into two groups. One group was fed with high-fat diet for 8 weeks and injected with streptozocin（ STZ） to create type 2 diabetic rat models. The other group was fed with normal diet to serve as controls. All mice were killed and corpus cavernosum tissues were harvested. After that H_2S concentrations in corpus cavernosum tissues were measured.The mRNA and protein expressions of CSE were detected with RT-PCR and Western blotting respectively. ResultsThe H_2S level in the corpus cavernosum tissues was significantly decreased in the diabetic group compared with the controls（ P〈0. 01）. The mRNA and protein expressions of CSE were significantly decreased in the diabetic group（ both P〈0. 05）. Conclusion H_2S concentration was significantly decreased in the corpus cavernosum tissues of diabetic rats,which may be associated with the down-regulation of mRNA and protein expressions of CSE.

关 键 词：糖尿病 勃起功能障碍 硫化氢 胱硫醚-Γ-裂解酶 大鼠 

分 类 号：R587.2[医药卫生—内分泌]