太赫兹波段超材料在核酸恒温指数扩增检测上的应用  

Detection of Isothermal Exponential Amplification Reaction Using Terahertz Metamaterials

作　　者：李永川[1] 王思江[2] 毛洪艳[2] 颜识涵[2] 刘毅[2] 魏东山[2] 夏良平[2] 黄庆[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]中国科学院重庆绿色智能技术研究院,跨尺度制造技术重庆市重点实验室,重庆400714

出　　处：《传感技术学报》2016年第9期1341-1344,共4页Chinese Journal of Sensors and Actuators

基　　金：国家重点基础研究发展计划项目(2015CB755400;2015CB755402);国家自然科学基金重点项目(81430054);物理与生物医学交叉实验室孵化基金项目(WSS-2012~501)

摘　　要：超材料对电磁场具有介电环境敏感和局域电场增强等奇特的电磁特性,近年来广泛用于无标记生物检测。文中设计并制作了一种金属开口谐振环阵列结构的超材料,在太赫兹波段下分别检测核酸恒温指数扩增前后反应体系,在氮气干燥环境下,扩增反应前后的频率偏移量分别为Δf1=(54±3)GHz、Δf2=(60±5)GHz。实验结果显示金属开口谐振环阵列结构的超材料可以作为一种生物传感器快速无标记检测核酸扩增前后的变化。Metamaterial has a dielectric environmentally sensitive and local electric-field enhancement in electro-magnetic field,It was widely used in label-free biodetection in recent years. We designed and fabricated a metama-terial structure assembled by split ring resonator. Before and after the isothermal exponential amplification reactionsystem were detected at the terahertz band. In dry nitrogen gas environment,the resonance frequency offset wereΔf1=（54±3）GHz and Δf2=（60±5）GHz at before and after reaction.The experimental result show that the metamateri-al assembled by split ring resonator can be used as a biosensor for rapid label-free detection in changes of beforeand after nucleic acid amplification.

关 键 词：太赫兹 超材料 金属开口谐振环阵列 

分 类 号：O456[理学—无线电物理]