中波紫外线对人永生化表皮细胞损伤作用  

Damage effect of ultraviolet B on HaCaT cells

作　　者：刘建宝[1] 侯海峰[2] 刘亚奇[2] 李群伟[2] 

机构地区：[1]济南市疾病预防控制中心,山东250000 [2]泰山医学院公共卫生学院

出　　处：《中国公共卫生》2016年第10期1330-1332,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81060228;81273155);山东省自然科学基金(ZR2012HL47)

摘　　要：目的探讨中波紫外线(UVB)对人永生化表皮(Ha Ca T)细胞的损伤作用,为紫外线损伤防护提供技术支持。方法取体外培养Ha Ca T细胞,用0(对照组)、10、30、60 m J/cm2剂量的UVB照射后继续培养12 h,以流式细胞术检测细胞凋亡率,用彗星实验(单细胞凝胶电泳)检测细胞DNA损伤情况。结果对照组、10、30、60 m J/cm2紫外线照射组Ha Ca T细胞早期凋亡率分别为(1.2±0.14)%、(2.23±0.37)%、(4.13±0.29)%、(5.97±0.17)%,晚期凋亡率分别为(2±0.13)%、(7.63±0.52)%、(23.36±0.98)%、(40.46±1.39)%,与对照组比较,UVB照射组Ha Ca T细胞早期、晚期凋亡率明显增加,差异有统计学意义(P<0.05);对照组、10、30、60 m J/cm2紫外线照射组Ha Ca T细胞DNA尾矩分别为(0.13±0.25)、(21.78±2.31)、(53.48±5.66)、(79.16±7.47),Olive尾矩分别为(0.32±0.47)、(16.17±1.96)、(36.43±2.89)、(51.71±1.87),与对照组比较,UVB照射组Ha Ca T细胞DNA尾矩、Olive尾矩明显延长,差异有统计学意义(P<0.05)。结论 UVB照射可使Ha Ca T细胞DNA产生损伤,诱发细胞凋亡,细胞DNA损伤可能是某些高原罕见病的发病原因之一。Objective To investigate the effect of ultraviolet B （UVB） on HaCaT ceUs for providing technical support to prevention of ultraviolet damage. Methods Cultured HaCaT ceils were exposed to UVB at intensity of 0,10,30, and 60 mJ/cm^2 and subsequently incubated for 12 hours. Flow cytometry （FCM） was used to detect proliferation rates of HaCaT ceils exposed to different intensities of UVB. Commet method was used to detect DNA damage of the HaCaT cells. Results The early apoptotic rates of control group, 10,30, and 60 mJ/cm^2 UVB groups were 1.2 ± 0. 14% ,2. 23 ±0. 37 % ,4. 13 ±0. 29% ,and 5.97 ±0. 17% ;the late apoptotic rates were 2 ±0. 13% ,7.63 ±0. 52% ,23.36 ±0. 98%, and 40.46 ± 1.39 %, with statistically significant difference （ P 〈 0.05 ）. The tail moments （TM） of control group, 10, 30,and 60 mJ/cm2 UVB groups were 0. 13 ± 0. 25,21.78 ± 2. 31,53.48 ± 5.66, and 79. 16 ±7.47;the olive tail moments （OTM） were 0. 32 ± 0.47,16. 17 ± 1.96,36.43 ± 2. 89, and 51.71± 1.87, with statistically significant difference （ P 〈 0.05 ）. Conclusion UVB can induce cell apoptosis and DNA damage and DNA damage may be one of the causes of some rare diseases among residents living in plateau area.

关 键 词：紫外线 HACAT细胞 细胞凋亡 DNA损伤 

分 类 号：R322.991[医药卫生—人体解剖和组织胚胎学]