新疆药桑叶中1-脱氧野尻霉素定性及定量分析  

Qualitative and Quantitative Analysis of 1-Deoxynojirimycin in Morus nigra Leaves of Xinjiang

作　　者：孙莲[1] 朱卫敏[1] 何悦[1] 李晓艳[1] 

机构地区：[1]新疆医科大学药学院化学教研室,新疆乌鲁木齐830054

出　　处：《中国现代中药》2016年第10期1291-1295,共5页Modern Chinese Medicine

摘　　要：目的：建立新疆药桑叶中1-脱氧野尻霉素（DNJ）的TLC定性鉴别及HPLC定量分析方法。方法：以正丁醇-冰醋酸-水（4．5：1．2：1．2）为展开剂，在高效硅胶G薄层色谱板上展开，氯-联甲苯胺为显色剂。采用YMC—pack ODS—A色谱柱（250mm×4．6mm，5μm），以甲醇（A）-0．1％醋酸水溶液（B）为流动相，等度洗脱（65％A，35％B），检测波长为263nm，流速为1．0mL·min-1。结果：新疆药桑叶中的DNJ在白光下检视，出现明显斑点，Rf值为0．41。DNJ的平均回收率为99．5％（RSD=1．3％），测得不同产地药桑叶中DNJ的含量为0．79—3．42mg·g-1。结论：建立的方法简便、可行、灵敏、专属性强，重复性好，可用于新疆药桑叶的质量控制。Objective： To establish a TLC identification and a HPLC quantitative analysis methods of 1- deoxynojirimycin （DNJ） in Morus nigra leaves of Xinjiang. Methods： DNJ was developed on efficient silica gel G plate using n-butanol-acetic acid-water （4.5：1.2：1.2） as a developing system, chloro-o-toluidine was used as a chromogenic reagent. The content determination was performed on a YMC pack ODS column （250 mm × 4. 6 mm, 5 μm） with methanol-0. 1% acetic acid solution as the mobile phase, isocratic elution（65% A, 35% B）, detection wavelength was 263 nm, and the flow rate was 1.0 mL ·min-l. Results： Obvious spots of DNJ appeared under natural light, its Rf = 0.41, the average recovery of DNJ of HPLC was 99. 5% （ RSD = 1.3% ）, and the content of DNJ from different area＇ s M. nigra leaves were 0. 79 3.42 mg· g- 1. Conclusion： The established method is simple, practical, sensitive, specific and reproducible, which can be used as a quality control of M. nigra leaves.

关 键 词：药桑叶 1-脱氧野尻霉素 薄层鉴别 高效液相色谱法 

分 类 号：R282.74[医药卫生—中药学]