不同苜蓿种质材料对茎点霉叶斑病的抗性评价  

Resistance Evaluation of Different Alfalfa Materials on Phoma Leaf Spot

作　　者：张丽[1,2] 潘龙其 袁庆华[1] 王瑜[1] 苗丽宏[1] ZHANG Li PAN Long-qi YUAN Qing-hua WANG Yu MIAO Li-hong(Institute of Animal Science of CAAS, Beijing 100193, China College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草地学报》2016年第3期652-657,共6页Acta Agrestia Sinica

基　　金："十二五"国家科技支撑计划课题(2011BAD17B01);公益性行业(农业)科研专项经费项目(201303057)资助

摘　　要：为了明确不同苜蓿材料（Medicago Sativa）对茎点霉叶斑病的抗性,对来自国内外的30份苜蓿材料进行了苗期抗病性评价,采用紫外分光光度计比色法,对不同抗感病材料体内的苯丙氨酸解氨酶（PAL）、过氧化氢酶（CAT）、过氧化物歧化酶（SOD）活性变化进行了研究。结果表明：不同苜蓿材料对茎点霉叶斑病的抗病性存在显著差异,各材料间病情指数（4.28-51.65）变化较大,其中抗病性最强的是来自内蒙的‘敖汉苜蓿’,感病性最强的是来自陕西的‘陕西苜蓿’,中抗材料有10份。接菌前,各材料间CAT,SOD活性差异显著（P〈0.05）,而PAL活性差异不显著;接菌后,3种酶活性均比接菌前有所升高,CAT和PAL呈先增后减的趋势,SOD活性呈依次递减趋势;同一材料在接菌后第4,8,12天3种酶活性也表现显著性差异（P〈0.05）。In order to define the resistance against alfalfa phoma leaf spot, the resistance evaluation was conducted with 30 alfalfa materials from China and abroad at seedling stage. Activities of phenylalanine ammonia lyase （PAL）, catalase （CAT） and superoxide dismutase （SOD） were studied by using ultraviolet spectrophotometer colorimetric method. The results showed that the resistances of different materials had marked differences against the alfalfa phoma leaf spot, the disease indexes of 30 materials were ranging from 4.28 to 51.65, of which Medicago sativa Aohan come from Inner Mongolia was highly resistant and M. sativa Shanxi come from Shanxi was highly sensitive, 10 cultivars were moderately resistant. The test of CAT and SOD activities showed significant differences among each materials before inoculation （P〈 0.05）, while PAL activities were not. The activities of three enzymes were increased after artificially inoc- ulated with the pathogen of Phoma medicaginis, CAT and PAL activities were first increased then decreased. SOD activities showed a descending trend. These enzymes activities had significantly different among the same material at the 4th, the 8th, the 12th days after inoculation （P〈0.05）.

关 键 词：苜蓿茎点霉叶斑病 抗病性 酶活性 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]