基于神经生物电原理治疗原发性痛经的作用机制研究  

Research of the therapy of the primary dysmenorrhea based on the principle of neurobiology electric

作　　者：李盈洁[1] 饶莹 曾茜[1] 胡析 王一枝[1] 易学良[1] 牛英杰[1] 张晓[1] Li Ying-Jie Rao Ying Zeng Xi Hu Xi Wang Yi-Zhi Yi Xue-Liang Niu Ying-Jie Zhang Xiao(ExperimentalTeaching Center of Basic Medical Science of Chengdu Medical College, Sichuan Chengdu 610500 People＇s Hospital of Mengzi City, Yunnan Mengzi 661100)

机构地区：[1]成都医学院基础医学实验教学中心,四川成都610500 [2]云南省蒙自市人民医院,云南蒙自661100

出　　处：《四川生理科学杂志》2016年第3期133-136,共4页Sichuan Journal of Physiological Sciences

基　　金：成都医学院大学生创新实验项目(编号:CX201213)

摘　　要：目的:探究神经生物电刺激对原发性痛经(Primary dysmenorrhea,PD)大鼠P物质(Substance P,SP)的表达变化的影响。方法:将SD大鼠随机分为非模型对照组、模型对照组和实验组3组。实验组给予股部皮下注射已烯雌酚,每天一次,连续10d,第1天0.8mg·只^(-1),第2-9天0.4mg·只^(-1),第10天0.8mg·只^(-1);第11天腹腔注射缩宫素2U·只^(-1);对实验组大鼠进行神经生物电刺激干预,并在1h、3h和7h后采用扭体评分进行运动评价。模型对照组只注射与实验组相同剂量的已烯雌酚与缩宫素,不进行神经生物电刺激干预,其余操作不变。非模型对照组不注射已烯雌酚与缩宫素,不进行神经生物电刺激干预,其余操作不变。免疫组织化学技术检测各组大鼠脊髓后角中SP的定位及表达变化,采用免疫荧光技术探究SP与脑源性神经营养因子(Brain derived neurotrophic factor,BDNF)在脊髓后角的共定位情况。结果:扭体评分显示实验组大鼠运动增加幅度明显小于模型对照组(P<0.05)。SP主要分布于脊髓后角Ⅱ板层,所有时间点实验组SP表达均低于模型对照组,在3h表达差异最大(P<0.05)。免疫荧光双标技术显示SP和BDNF在脊髓后角共表达。结论:神经生物电刺激能抑制SP的表达,影响原发性痛经,其机制可能与SP及BDNF共同作用有关。Objective：To explore the influence of substance P（SP）on rats with primary dysmenorrhea after electric therapy.Methods：SD rats were divided into 3groups：control group,model group and experimental group.Rats in experimental group were given diethylstilbestrol injection in subcutaneous tissue of femoral region once a day for 10 days.Each rat was given 0.8mg diethylstilbestrol injection at the first day,0.4mg diethylstilbestrol injection from second day to ninth day,0.8mg diethylstilbestrol injection on ten day.Each rat was given 2 Uoxytocin by intraperitoneal injection on the eleventh day.Rats in the experimental group were given the intervention of neural bioelectricity stimulation and evaluated by the twisting reaction scores in 1h,3h,and 7hafter the intervention.Rats in model group were given the same diethylstilbestrol injection and oxytocin as the rats in experimental group,not the intervention of neural bioelectricity stimulation.The remaming operations in model of group were also the same as that in experimental group.Rats in control group were not given the diethylstilbestrol injection and oxytocin or the intervention of neural bioelectricity stimulation.The remaming operations in model of group were also the same as that in experimental group.In addition,immunohistochemistry was used to observe the distribution and the expression of SP in the posterior horn of the spinal cord.Immunofluorescence was used to detect whether SP and brain derived neurotrophic factor（BDNF）co-expressed in the posterior horn of the spinal cord.Results：Twisting reaction scores in the experimental group were significantly less than that in the model group（P〈0.05）.Immunohistochemistry results showed that SP was mainly located in the second layer of posterior horn of spinal cord.Positive cells in the experimental group were always less than that in the model group,and the expression at 3hhad the most significantly difference between the two groups（P〈0.05）.Immunofluorescence indicated that SP and BDNF were co

关 键 词：原发性痛经 神经生物电刺激 P物质 脑源性神经营养因子 

分 类 号：R711.51[医药卫生—妇产科学]