伤寒沙门菌外膜孔道蛋白ompC、ompF缺陷株的制备及其耐药性  

Construction of ompC and ompF deletion mutant in Salmonella enterica serovar Typhi and their participation in drug-resisitance

作　　者：汪伟伟[1] 夏歆 王静瑜[1] 张桂红[1] 陈龙[1] 黄新祥[1] 生秀梅[1] WANG Wei-wei XIA Xin WANG Jing-yu ZHANG Gui-hong CHEN Long HUANG Xin-xiang SHENG Xiu-mei(School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)

机构地区：[1]江苏大学医学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2015年第6期532-535,542,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(31000046);江苏大学高级人才启动基金资助项目(11JD063);国家博士后基金资助项目(2015M571702)

摘　　要：目的:制备伤寒沙门菌omp C缺陷株及omp C-omp F双缺陷株,研究外膜孔道蛋白Omp C、Omp F在伤寒沙门菌耐药中的作用。方法:经自杀质粒介导的同源重组方法制备omp C缺陷株及omp C-omp F双缺陷株,比较伤寒沙门菌omp C缺陷株、omp F缺陷株及omp C-omp F双缺陷株与野生株在多黏菌素B、卡那霉素以及氨苄青霉素处理下的生存能力。结果:成功制备伤寒沙门菌omp C单缺陷以及omp C-omp F双缺陷株。各菌株在普通培养条件下生长情况无差异;多黏菌素B及氨青苄霉素处理条件下omp C缺陷株、omp C-omp F双缺陷株生存能力明显强于野生株,而omp F缺陷株生存能力则低于野生株;卡那霉素处理条件下,各缺陷株生存能力均明显低于野生株。结论:外膜孔道蛋白Omp C、Omp F参与伤寒沙门菌耐药,但对不同药物的耐药机制不尽相同,具体机制有待进一步研究。Objective： To construct ompC deletion mutant and ompC-ompF double deletion mutant, and investigate the role of outer membrane protein 0mpC and 0mpF in drug-resistance of S. Typhi. Methods： The ompC deletion mutant and the ompC-ompF double deletion mutant of S. Typhi were prepared by the method of homologous recombination mediated by suicide plasmid. The growth ability of the wild type, ompC deletion mutant, ompF deletion mutant, and ompC-ompF double deletion mutant under polymyxin B, Kanamycin and Ampicillin treatment were performed. Results： The ompC deletion mutant and the ompC- ompF double deletion mutant of S. Typhi were successfully constructed. The results showed that no signifi- cant difference was observed between these strains under common conditions. However, under polymyxin B and Ampicillin treatment, the growth of ompC deletion mutant and ompC-ompF double deletion mutant were much higher than that of the wild type strain, while the growth of ompF deletion mutant was slower than that of the wild type strain. Under Kanamycin treatment, the growth of all the mutants was significantly slower than that of the wild type strain. Conclusion： OmpC and OmpF are involved in the drug-resistance of S. Typhi, and the mechanisms are different between different kinds of drugs, which need further research.

关 键 词：伤寒沙门菌 OMPC OMPF 抗生素 耐药性 

分 类 号：R378.22[医药卫生—病原生物学]